TY - JOUR
T1 - Inorganic nanoparticles kill Toxoplasma gondii via changes in redox status and mitochondrial membrane potential
AU - Adeyemi, Oluyomi Stephen
AU - Murata, Yuho
AU - Sugi, Tatsuki
AU - Kato, Kentaro
N1 - Funding Information:
The study was funded through a Japan Society for the Promotion of Science (JSPS) Fellowship to Dr OS Adeyemi. This study was supported by grants-in-aid for Young Scientists, Exploratory Research, and Scientific Research on Innovative Areas (3308 and 3407) from the Ministry of Education, Culture, Science, Sports, and Technology (MEXT) of Japan; by the Program to Disseminate Tenure Tracking System and the Adaptable & Seamless Technology Transfer Program through Target-driven R&D (A-STEP) from the Japan Science and Technology Agency (JST); by the Ito Foundation; and by the Promotion for Young Research Talent and Network from Northern Advancement Center for Science & Technology (NOASTEC). The authors appreciate Dr Daisuke Kondoh of the Obihiro University of Agriculture and Veterinary Medicine, Japan, for providing assistance with electron microscopy.
Publisher Copyright:
© 2017 Adeyemi et al.
PY - 2017/2/28
Y1 - 2017/2/28
N2 - This study evaluated the anti-Toxoplasma gondii potential of gold, silver, and platinum nanoparticles (NPs). Inorganic NPs (0.01-1,000 µg/mL) were screened for antiparasitic activity. The NPs caused >90% inhibition of T. gondii growth with EC50 values of ≤7, ≤1, and ≤100 µg/mL for gold, silver, and platinum NPs, respectively. The NPs showed no host cell cytotoxicity at the effective anti-T. gondii concentrations; the estimated selectivity index revealed a ≥20-fold activity toward the parasite versus the host cell. The anti-T. gondii activity of the NPs, which may be linked to redox signaling, affected the parasite mitochondrial membrane potential and parasite invasion, replication, recovery, and infectivity potential. Our results demonstrated the antiparasitic potential of NPs. The findings support the further exploration of NPs as a possible source of alternative and effective anti-T. gondii agents.
AB - This study evaluated the anti-Toxoplasma gondii potential of gold, silver, and platinum nanoparticles (NPs). Inorganic NPs (0.01-1,000 µg/mL) were screened for antiparasitic activity. The NPs caused >90% inhibition of T. gondii growth with EC50 values of ≤7, ≤1, and ≤100 µg/mL for gold, silver, and platinum NPs, respectively. The NPs showed no host cell cytotoxicity at the effective anti-T. gondii concentrations; the estimated selectivity index revealed a ≥20-fold activity toward the parasite versus the host cell. The anti-T. gondii activity of the NPs, which may be linked to redox signaling, affected the parasite mitochondrial membrane potential and parasite invasion, replication, recovery, and infectivity potential. Our results demonstrated the antiparasitic potential of NPs. The findings support the further exploration of NPs as a possible source of alternative and effective anti-T. gondii agents.
KW - Antiparasite
KW - Drug screening
KW - Nanomedicine
KW - Toxoplasmosis
UR - http://www.scopus.com/inward/record.url?scp=85014404695&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85014404695&partnerID=8YFLogxK
U2 - 10.2147/IJN.S122178
DO - 10.2147/IJN.S122178
M3 - Article
C2 - 28280332
AN - SCOPUS:85014404695
SN - 1176-9114
VL - 12
SP - 1647
EP - 1661
JO - International Journal of Nanomedicine
JF - International Journal of Nanomedicine
ER -