Insect pheromone behavior: Fruit fly

Daisuke Yamamoto, Soh Kohatsu, Masayuki Koganezawa

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

2 Citations (Scopus)

Abstract

The amenability to genetics of Drosophila melanogaster has made this organism one of the best-suited models for studying the neurobiology of pheromone-guided behavior. Single-male assays use the minigene encoding the thermosensitive channel dTrpA1 to activate neurons expressing fruitless (fru), a major courtship regulator gene, and thereby induce most of the elementary courtship acts in a solitary male exposed to temperature increase. Tethered male assays allow Ca2+-imaging of neuronal activities of a male fly displaying courtship behavior on a treadmill when stimulated with a female or pheromones. Here we describe technical details of these assays.

Original languageEnglish
Title of host publicationPheromone Signaling
Subtitle of host publicationMethods and Protocols
EditorsKazushige Touhara
Pages261-272
Number of pages12
DOIs
Publication statusPublished - 2013

Publication series

NameMethods in Molecular Biology
Volume1068
ISSN (Print)1064-3745

Keywords

  • Ca-imaging
  • Courtship
  • Drosophila
  • fruitless
  • MARCM
  • Thermogenetics

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