Insect pheromone behavior: Fruit fly

Daisuke Yamamoto, Soh Kohatsu, Masayuki Koganezawa

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

2 Citations (Scopus)


The amenability to genetics of Drosophila melanogaster has made this organism one of the best-suited models for studying the neurobiology of pheromone-guided behavior. Single-male assays use the minigene encoding the thermosensitive channel dTrpA1 to activate neurons expressing fruitless (fru), a major courtship regulator gene, and thereby induce most of the elementary courtship acts in a solitary male exposed to temperature increase. Tethered male assays allow Ca2+-imaging of neuronal activities of a male fly displaying courtship behavior on a treadmill when stimulated with a female or pheromones. Here we describe technical details of these assays.

Original languageEnglish
Title of host publicationPheromone Signaling
Subtitle of host publicationMethods and Protocols
EditorsKazushige Touhara
Number of pages12
Publication statusPublished - 2013

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Ca-imaging
  • Courtship
  • Drosophila
  • fruitless
  • Thermogenetics


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