Insect resistant rice generated by introduction of a modified δ-endotoxin gene of Bacillus thuringiensis

Hideya Fujimoto; Kimiko Itoh; Mikihiro Yamamoto; Junko Kyozuka; Ko Shimamoto

doi:10.1038/nbt1093-1151

Insect resistant rice generated by introduction of a modified δ-endotoxin gene of Bacillus thuringiensis

Hideya Fujimoto, Kimiko Itoh, Mikihiro Yamamoto, Junko Kyozuka, Ko Shimamoto

Research output: Contribution to journal › Article › peer-review

213 Citations (Scopus)

Abstract

As a first step towards development of insect resistant rice we have introduced a truncated δ-endotoxin gene, cryIA(b) of Bacillus thuringiensis (B.t.) which has specific biological activity against lepidopteran insects into a japonica rice. To highly express the cryIA(b) gene in rice the coding sequence was extensively modified based on the codon usage of rice genes. Transgenic plants efficiently expressed the modified cryIA(b) gene at both mRNA and protein levels. Bioassays using R₂ generation plants with two major rice insect pests, striped stemborer (Chilo suppressalis) and leaffolder (Cnaphalocrosis medinalis), indicated that transgenic rice plants expressing the CryIA(b) protein are more resistant to these pests than untransformed control plants. Our results suggest that the B.t. endotoxin genes will be useful for the rational development of new rice varieties resistant to major insect pests.

Original language	English
Pages (from-to)	1151-1155
Number of pages	5
Journal	Nature Biotechnology
Volume	11
Issue number	10
DOIs	https://doi.org/10.1038/nbt1093-1151
Publication status	Published - 1993 Jan 1
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology
Molecular Medicine
Biomedical Engineering

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abstract = "As a first step towards development of insect resistant rice we have introduced a truncated δ-endotoxin gene, cryIA(b) of Bacillus thuringiensis (B.t.) which has specific biological activity against lepidopteran insects into a japonica rice. To highly express the cryIA(b) gene in rice the coding sequence was extensively modified based on the codon usage of rice genes. Transgenic plants efficiently expressed the modified cryIA(b) gene at both mRNA and protein levels. Bioassays using R2 generation plants with two major rice insect pests, striped stemborer (Chilo suppressalis) and leaffolder (Cnaphalocrosis medinalis), indicated that transgenic rice plants expressing the CryIA(b) protein are more resistant to these pests than untransformed control plants. Our results suggest that the B.t. endotoxin genes will be useful for the rational development of new rice varieties resistant to major insect pests.",

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AU - Itoh, Kimiko

AU - Yamamoto, Mikihiro

AU - Kyozuka, Junko

AU - Shimamoto, Ko

PY - 1993/1/1

Y1 - 1993/1/1

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Insect resistant rice generated by introduction of a modified δ-endotoxin gene of Bacillus thuringiensis

Abstract

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