Integration of antibody arrays into a preassembled microfluidic device for on-demand immunoassay

Masahiko Hashimoto; Hirokazu Kaji; Maria E. Kemppinen; Matsuhiko Nishizawa

Integration of antibody arrays into a preassembled microfluidic device for on-demand immunoassay

Masahiko Hashimoto, Hirokazu Kaji, Maria E. Kemppinen, Matsuhiko Nishizawa

ENG - Finemechanics

Tohoku University

Research output: Contribution to conference › Paper › peer-review

Abstract

This paper describes a method to create a patterned surface within a microchannel by electrochemical means, which enables on-demand and site-specific immobilization of protein array after the device is fully assembled. High density protein immobilization is achieved by locating proteins to a three-dimensional microstructure. In addition, by first depositing a pattern of poly(ethylene glycol) (PEG)-based copolymer in the microchannel, a uniform protein pattern is obtained even in a stream flow.

Original language	English
Pages	846-848
Number of pages	3
Publication status	Published - 2006
Event	10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan Duration: 2006 Nov 5 → 2006 Nov 9

Conference

Conference	10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006
Country/Territory	Japan
City	Tokyo
Period	06/11/5 → 06/11/9

Keywords

3D microstructures
Electrochemical method
Microchannel
Protein pattern

Cite this

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title = "Integration of antibody arrays into a preassembled microfluidic device for on-demand immunoassay",

abstract = "This paper describes a method to create a patterned surface within a microchannel by electrochemical means, which enables on-demand and site-specific immobilization of protein array after the device is fully assembled. High density protein immobilization is achieved by locating proteins to a three-dimensional microstructure. In addition, by first depositing a pattern of poly(ethylene glycol) (PEG)-based copolymer in the microchannel, a uniform protein pattern is obtained even in a stream flow.",

keywords = "3D microstructures, Electrochemical method, Microchannel, Protein pattern",

author = "Masahiko Hashimoto and Hirokazu Kaji and Kemppinen, {Maria E.} and Matsuhiko Nishizawa",

year = "2006",

language = "English",

pages = "846--848",

note = "10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 ; Conference date: 05-11-2006 Through 09-11-2006",

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Integration of antibody arrays into a preassembled microfluidic device for on-demand immunoassay. / Hashimoto, Masahiko; Kaji, Hirokazu; Kemppinen, Maria E. et al.
2006. 846-848 Paper presented at 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006, Tokyo, Japan.

Research output: Contribution to conference › Paper › peer-review

TY - CONF

T1 - Integration of antibody arrays into a preassembled microfluidic device for on-demand immunoassay

AU - Hashimoto, Masahiko

AU - Kaji, Hirokazu

AU - Kemppinen, Maria E.

AU - Nishizawa, Matsuhiko

PY - 2006

Y1 - 2006

N2 - This paper describes a method to create a patterned surface within a microchannel by electrochemical means, which enables on-demand and site-specific immobilization of protein array after the device is fully assembled. High density protein immobilization is achieved by locating proteins to a three-dimensional microstructure. In addition, by first depositing a pattern of poly(ethylene glycol) (PEG)-based copolymer in the microchannel, a uniform protein pattern is obtained even in a stream flow.

AB - This paper describes a method to create a patterned surface within a microchannel by electrochemical means, which enables on-demand and site-specific immobilization of protein array after the device is fully assembled. High density protein immobilization is achieved by locating proteins to a three-dimensional microstructure. In addition, by first depositing a pattern of poly(ethylene glycol) (PEG)-based copolymer in the microchannel, a uniform protein pattern is obtained even in a stream flow.

KW - 3D microstructures

KW - Electrochemical method

KW - Microchannel

KW - Protein pattern

UR - http://www.scopus.com/inward/record.url?scp=84902491918&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84902491918&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:84902491918

SP - 846

EP - 848

T2 - 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006

Y2 - 5 November 2006 through 9 November 2006

ER -

Integration of antibody arrays into a preassembled microfluidic device for on-demand immunoassay

Abstract

Conference

Keywords

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