Integrator complex plays an essential role in adipose differentiation

Yuichiro Otani, Yusuke Nakatsu, Hideyuki Sakoda, Toshiaki Fukushima, Midori Fujishiro, Akifumi Kushiyama, Hirofumi Okubo, Yoshihiro Tsuchiya, Haruya Ohno, Shin Ichiro Takahashi, Fusanori Nishimura, Hideaki Kamata, Hideki Katagiri, Tomoichiro Asano

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)


The dynamic process of adipose differentiation involves stepwise expressions of transcription factors and proteins specific to the mature fat cell phenotype. In this study, it was revealed that expression levels of IntS6 and IntS11, subunits of the Integrator complex, were increased in 3T3-L1 cells in the period when the cells reached confluence and differentiated into adipocytes, while being reduced to basal levels after the completion of differentiation. Suppression of IntS6 or IntS11 expression using siRNAs in 3T3-L1 preadipocytes markedly inhibited differentiation into mature adipocytes, based on morphological findings as well as mRNA analysis of adipocyte-specific genes such as Glut4, perilipin and Fabp4. Although Pparγ2 protein expression was suppressed in IntS6 or IntS11-siRNA treated cells, adenoviral forced expression of Pparγ2 failed to restore the capacity for differentiation into mature adipocytes. Taken together, these findings demonstrate that increased expression of Integrator complex subunits is an indispensable event in adipose differentiation. Although further study is necessary to elucidate the underlying mechanism, the processing of U1, U2 small nuclear RNAs may be involved in cell differentiation steps.

Original languageEnglish
Pages (from-to)197-202
Number of pages6
JournalBiochemical and Biophysical Research Communications
Issue number2
Publication statusPublished - 2013 May 3


  • Adipose differentiation
  • Integrator complex
  • IntS11
  • IntS6


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