TY - JOUR
T1 - Interaction between leptospiral lipopolysaccharide and toll-like receptor 2 in pig fibroblast cell line, and inhibitory effect of antibody against leptospiral lipopolysaccharide on interaction
AU - Guo, Yijie
AU - Fukuda, Tomokazu
AU - Nakamura, Shuichi
AU - Bai, Lanlan
AU - Xu, Jun
AU - Kuroda, Kengo
AU - Tomioka, Rintaro
AU - Yoneyama, Hiroshi
AU - Isogai, Emiko
N1 - Publisher Copyright:
Copyright © 2015 by Asian-Australasian Journal of Animal Sciences
PY - 2015/2/1
Y1 - 2015/2/1
N2 - Leptospiral lipopolysaccharide (L-LPS) has shown potency in activating toll-like receptor 2 (TLR2) in pig fibroblasts (PEFs-NCC1), and causes the expression of proinflammatory cytokines. However, the stimulation by L-LPS was weak eliciting the function of TLR2 sufficiently in pig innate immunity responses during Leptospira infection. In this study, the immune response of pig embryonic fibroblast cell line (PEFs-SV40) was investigated and was found to be the high immune response, thus TLR2 is the predominate receptor of L-LPS in pig cells. Further, we found a strategy using the antibody against L-LPS, to prevent L-LPS interaction with TLR2 in pig cells which could impact on immune activation.
AB - Leptospiral lipopolysaccharide (L-LPS) has shown potency in activating toll-like receptor 2 (TLR2) in pig fibroblasts (PEFs-NCC1), and causes the expression of proinflammatory cytokines. However, the stimulation by L-LPS was weak eliciting the function of TLR2 sufficiently in pig innate immunity responses during Leptospira infection. In this study, the immune response of pig embryonic fibroblast cell line (PEFs-SV40) was investigated and was found to be the high immune response, thus TLR2 is the predominate receptor of L-LPS in pig cells. Further, we found a strategy using the antibody against L-LPS, to prevent L-LPS interaction with TLR2 in pig cells which could impact on immune activation.
KW - Antibody against leptospiral lipopolysaccharide
KW - Cytokines
KW - Leptospiral lipopolysaccharide
KW - Pig fibroblast cell line
KW - Toll-like receptor 2
UR - http://www.scopus.com/inward/record.url?scp=84920171923&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84920171923&partnerID=8YFLogxK
U2 - 10.5713/ajas.14.0440
DO - 10.5713/ajas.14.0440
M3 - Article
AN - SCOPUS:84920171923
SN - 1011-2367
VL - 28
SP - 273
EP - 279
JO - Asian-Australasian Journal of Animal Sciences
JF - Asian-Australasian Journal of Animal Sciences
IS - 2
ER -