Intracellular Ca2+ imaging for micropatterned cardiac myocytes

Hirokazu Kaji, Kimiyasu Takoh, Matsuhiko Nishizawa, Tomokazu Matsue

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)


The patterning of cardiac myocytes on a micron scale (-5 pm) was achieved by microcontact printing of fibronectin onto a hydrophobically pretreated glass substrate. The patterned cardiac myocytes conjugated with each other by forming a gap junction, as judged from the synchronized Ca2+ transition over the pattern, and thus simultaneously contracted. The dynamic change of the Ca2+ concentration within the patterned tissue was analyzed quantitatively during successive contraction and relaxation using a Nipkow-type high-speed confocal microscope.

Original languageEnglish
Pages (from-to)748-751
Number of pages4
JournalBiotechnology and Bioengineering
Issue number6
Publication statusPublished - 2003 Mar 20


  • Ca imaging
  • Cardiac myocytes
  • Confocal microscope
  • Microcontact printing (pCP)
  • Pattering


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