TY - JOUR
T1 - Intrascleral transplantation of a collagen sheet with cultured brain-derived neurotrophic factor expressing cells partially rescues the retina from damage due to acute high intraocular pressure
AU - Abe, Toshiaki
AU - Tokitaish-Ikawa, Yumi
AU - Onami, Hideyuki
AU - Katsukura, Yuki
AU - Kaji, Hirokazu
AU - Nishizawa, Matsuhiko
AU - Nagai, Nobuhiro
N1 - Funding Information:
This study was supported by Health Labour Sciences Research Grant from the Ministry of Health Labour and Welfare (H23-kankaku-ippan-004, H24-nanchitoh-ippan-067).
Publisher Copyright:
© Springer Science+Business Media, LLC 2014.
PY - 2014
Y1 - 2014
N2 - We constructed brain-derived neurotrophic factor (BDNF) expressing rat retinal pigment epithelial (RPE) cells by stable transfection of BDNF cDNA, and the RPE cells were cultured on a cross-linked collagen sheet (Coll-RPE-BDNF). BDNF expression of the Coll-RPE-BDNF was confirmed by western blot, and the Coll-RPE-BDNF was transplanted into the rabbit sclera. In vivo BDNF expression was confirmed by His expression that was linked to the expressing BDNF. The effect of the released BDNF was examined in a rabbit acute high intraocular pressure system by electroretinogram and histological examination. Statistically significant preservation of ERG b wave amplitude was observed in the rabbits treated by Coll-RPE-BDNF when compared to that of no treatment. Statistically significant preservation of the thickness of the inner nuclear layer at the transplanted area was observed in the rabbits treated by Coll-RPE-BDNF compared to that of no treatment. Intra-scleral Coll-RPE-BDNF transplantation may partially rescue retinal cells from acute high intraocular pressure.
AB - We constructed brain-derived neurotrophic factor (BDNF) expressing rat retinal pigment epithelial (RPE) cells by stable transfection of BDNF cDNA, and the RPE cells were cultured on a cross-linked collagen sheet (Coll-RPE-BDNF). BDNF expression of the Coll-RPE-BDNF was confirmed by western blot, and the Coll-RPE-BDNF was transplanted into the rabbit sclera. In vivo BDNF expression was confirmed by His expression that was linked to the expressing BDNF. The effect of the released BDNF was examined in a rabbit acute high intraocular pressure system by electroretinogram and histological examination. Statistically significant preservation of ERG b wave amplitude was observed in the rabbits treated by Coll-RPE-BDNF when compared to that of no treatment. Statistically significant preservation of the thickness of the inner nuclear layer at the transplanted area was observed in the rabbits treated by Coll-RPE-BDNF compared to that of no treatment. Intra-scleral Coll-RPE-BDNF transplantation may partially rescue retinal cells from acute high intraocular pressure.
KW - Acute high ocular pressure
KW - Brain-derived neurotrophic factor
KW - Collagen sheet
KW - Sclera
KW - Transplantation
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U2 - 10.1007/978-1-4614-3209-8_105
DO - 10.1007/978-1-4614-3209-8_105
M3 - Article
C2 - 24664778
AN - SCOPUS:84904811400
SN - 0065-2598
VL - 801
SP - 837
EP - 843
JO - Advances in Experimental Medicine and Biology
JF - Advances in Experimental Medicine and Biology
ER -