TY - JOUR
T1 - Intratumoral Estrogen Concentration and Expression of Estrogen-Induced Genes in Male Breast Carcinoma
T2 - Comparison with Female Breast Carcinoma
AU - Takagi, Kiyoshi
AU - Moriya, Takuya
AU - Kurosumi, Masafumi
AU - Oka, Kimako
AU - Miki, Yasuhiro
AU - Ebata, Akiko
AU - Toshima, Takashi
AU - Tsunekawa, Shoji
AU - Takei, Hiroyuki
AU - Hirakawa, Hisashi
AU - Ishida, Takanori
AU - Hayashi, Shin ichi
AU - Kurebayashi, Junichi
AU - Sasano, Hironobu
AU - Suzuki, Takashi
N1 - Funding Information:
Funding This work was partly supported by Grant-in-Aid for Scientific Research (24790343) from the Japanese Ministry of Education, Culture, Sports, Science and Technology.
PY - 2013/2
Y1 - 2013/2
N2 - It is speculated that estrogens play important roles in the male breast carcinoma (MBC) as well as the female breast carcinoma (FBC). However, estrogen concentrations or molecular features of estrogen actions have not been reported in MBC, and biological significance of estrogens remains largely unclear in MBC. Therefore, we examined intratumoral estrogen concentrations, estrogen receptor (ER) α/ERβ status, and expression profiles of estrogen-induced genes in MBC tissues, and compared these with FBC. 17β-Estradiol concentration in MBC (n = 4) was significantly (14-fold) higher than that in non-neoplastic male breast (n = 3) and tended to be higher than that in FBC (n = 7). Results of microarray analysis clearly demonstrated that expression profiles of the two gene lists, which were previously reported as estrogen-induced genes in MCF-7 breast carcinoma cell line, were markedly different between MBC and FBC. In the immunohistochemistry, MBC tissues were frequently positive for aromatase (63 %) and 17β-hydroxysteroid dehydrogenase type 1 (67 %), but not for steroid sulfatase (6. 7 %). A great majority (77 %) of MBC showed positive for both ERα and ERβ, and its frequency was significantly higher than FBC cases. These results suggest that estradiol is locally produced in MBC tissue by aromatase. Different expression profiles of the estrogen-induced genes may associate with different estrogen functions in MBC from FBC, which may be partly due to their ERα/ERβ status.
AB - It is speculated that estrogens play important roles in the male breast carcinoma (MBC) as well as the female breast carcinoma (FBC). However, estrogen concentrations or molecular features of estrogen actions have not been reported in MBC, and biological significance of estrogens remains largely unclear in MBC. Therefore, we examined intratumoral estrogen concentrations, estrogen receptor (ER) α/ERβ status, and expression profiles of estrogen-induced genes in MBC tissues, and compared these with FBC. 17β-Estradiol concentration in MBC (n = 4) was significantly (14-fold) higher than that in non-neoplastic male breast (n = 3) and tended to be higher than that in FBC (n = 7). Results of microarray analysis clearly demonstrated that expression profiles of the two gene lists, which were previously reported as estrogen-induced genes in MCF-7 breast carcinoma cell line, were markedly different between MBC and FBC. In the immunohistochemistry, MBC tissues were frequently positive for aromatase (63 %) and 17β-hydroxysteroid dehydrogenase type 1 (67 %), but not for steroid sulfatase (6. 7 %). A great majority (77 %) of MBC showed positive for both ERα and ERβ, and its frequency was significantly higher than FBC cases. These results suggest that estradiol is locally produced in MBC tissue by aromatase. Different expression profiles of the estrogen-induced genes may associate with different estrogen functions in MBC from FBC, which may be partly due to their ERα/ERβ status.
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U2 - 10.1007/s12672-012-0126-6
DO - 10.1007/s12672-012-0126-6
M3 - Article
C2 - 23096432
AN - SCOPUS:84872282341
SN - 1868-8497
VL - 4
SP - 1
EP - 11
JO - Hormones and Cancer
JF - Hormones and Cancer
IS - 1
ER -
