TY - JOUR
T1 - Intrinsic Projections of Layer Vb Neurons to Layers Va, III, and II in the Lateral and Medial Entorhinal Cortex of the Rat
AU - Ohara, Shinya
AU - Onodera, Mariko
AU - Simonsen, Øyvind W.
AU - Yoshino, Rintaro
AU - Hioki, Hiroyuki
AU - Iijima, Toshio
AU - Tsutsui, Ken Ichiro
AU - Witter, Menno P.
N1 - Funding Information:
We thank Dr. Satoshi Inoue (National Institute of Infectious Diseases, Japan) for kindly supplying the monospecific rabbit anti-N antiserum and Dr. Kinjiro Morimoto (National Institute of Infectious Diseases, Japan) for kindly supplying the rabies virus (challenge virus standard strain). This study was supported by Grants-in-Aid for Scientific Research on Innovative Areas (16H01495) and by Grant-in-Aid for Scientific Research (KAKENHI) 15K18358 from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan. It was also supported by the Research Council of Norway: the Centre of Excellence scheme-Centre for Neural Computation (grant no. 223262), the National Infrastructure scheme–NORBRAIN (grant no. 197467), research grant no. 227769, and The Egil and Pauline Braathen and Fred Kavli Centre for Cortical Microcircuits. This study was partially supported by MEXT/JSPS (16H04663, 17K19451, 15H01430, and 16H01426) and by Brain/MINDS from AMED (JP18dm0207064 to H.H.).
Funding Information:
We thank Dr. Satoshi Inoue (National Institute of Infectious Diseases, Japan) for kindly supplying the monospecific rabbit anti-N antiserum and Dr. Kinjiro Morimoto (National Institute of Infectious Diseases, Japan) for kindly supplying the rabies virus (challenge virus standard strain). This study was supported by Grants-in-Aid for Scientific Research on Innovative Areas ( 16H01495 ) and by Grant-in-Aid for Scientific Research (KAKENHI) 15K18358 from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan . It was also supported by the Research Council of Norway : the Centre of Excellence scheme-Centre for Neural Computation (grant no. 223262 ), the National Infrastructure scheme–NORBRAIN (grant no. 197467), research grant no. 227769 , and The Egil and Pauline Braathen and Fred Kavli Centre for Cortical Microcircuits . This study was partially supported by MEXT /JSPS ( 16H04663 , 17K19451 , 15H01430 , and 16H01426 ) and by Brain/MINDS from AMED ( JP18dm0207064 to H.H.).
Publisher Copyright:
© 2018 The Authors
PY - 2018/7/3
Y1 - 2018/7/3
N2 - Layer V of the entorhinal cortex (EC) receives input from the hippocampus and originates main entorhinal outputs. The deep-sublayer Vb, immunopositive for the transcription factor Ctip2, is thought to be the main recipient of hippocampal projections, whereas the superficial-sublayer LVa, immunonegative for Ctip2, originates the main outputs of EC. This disrupts the proposed role of EC as mediating hippocampal-cortical interactions. With the use of specific (trans)synaptic tracing approaches, we report that, in medial entorhinal cortex, layer Vb neurons innervate neurons in layers Va, II, and III. A similar circuitry exists in the lateral entorhinal cortex. We conclude that EC-layer Vb neurons mediate two circuits in the hippocampus-memory system: (1) a hippocampal output circuit to telencephalic areas by projecting to layer Va and (2) a feedback projection, sending information back to the EC-hippocampal loop via neurons in layers II and III.
AB - Layer V of the entorhinal cortex (EC) receives input from the hippocampus and originates main entorhinal outputs. The deep-sublayer Vb, immunopositive for the transcription factor Ctip2, is thought to be the main recipient of hippocampal projections, whereas the superficial-sublayer LVa, immunonegative for Ctip2, originates the main outputs of EC. This disrupts the proposed role of EC as mediating hippocampal-cortical interactions. With the use of specific (trans)synaptic tracing approaches, we report that, in medial entorhinal cortex, layer Vb neurons innervate neurons in layers Va, II, and III. A similar circuitry exists in the lateral entorhinal cortex. We conclude that EC-layer Vb neurons mediate two circuits in the hippocampus-memory system: (1) a hippocampal output circuit to telencephalic areas by projecting to layer Va and (2) a feedback projection, sending information back to the EC-hippocampal loop via neurons in layers II and III.
KW - hippocampal-cortical connections
KW - hippocampal-entorhinal connections
KW - interlaminar connectivity
KW - parahippocampus
KW - rodent
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U2 - 10.1016/j.celrep.2018.06.014
DO - 10.1016/j.celrep.2018.06.014
M3 - Article
C2 - 29972772
AN - SCOPUS:85049016375
SN - 2211-1247
VL - 24
SP - 107
EP - 116
JO - Cell Reports
JF - Cell Reports
IS - 1
ER -