Investigation of biocompatibility on nitrogen-doped a-C:H film coating scaffold surface in in-vivo and in-vitro tests

Yasuharu Ohgoe, Tomoaki Wada, Yasuyuki Shiraishi, Hidekazu Miura, Kenji K. Hirakuri, Akio Funakubo, Tomoyuki Yambe, Yasuhiro Fukui

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

2 Citations (Scopus)


In this study, in order to investigate biocompatibility of nitrogen-doped hydrogenated amorphous carbon (a-C:H:N) film coating segmented polyurethane (SPU) scaffold fiber sheet (a-C:H:N-Scaffold) in in-vitro test, mouse fibroblasts (NIH 3T3) cells were grown on the a-C:H:N-Scaffold. The cell behavior was monitored by time-lapse imaging system. Additionally, the a-C:H:N-Scaffold was implanted at partial aorta descendens of a goat for 35 days. The surface morphology, composition, and wettability of the a-C:H:N-scaffold was estimated by Scanning Electron Microscope (SEM), X-ray photoelectron spectrometer (XPS), and contact angle measurement. In in-vitro test, it was observed that a-C:H:N film coating had a facilitatory effect on cell motility and cell growth. In in-vivo test, it was observed that the a-C:H:N-Scaffold surface was uniformly covered by neointima. The a-C:H:N-Scaffold surface had no thrombus formation as an inflammatory reaction and it was shown that the a-C:H:N film coating had a good blood compatibility. These results suggest that a-C:H:N film coating has good cytocompatibility and blood compatibility and it is a promising approach for improvement of biocompatibility of biomaterial surfaces.

Original languageEnglish
Title of host publicationBiomimetic, Bio-Inspired and Self-Assembled Materials for Engineered Surfaces and Applications
Number of pages6
Publication statusPublished - 2013
Event2012 MRS Fall Meeting - Boston, MA, United States
Duration: 2012 Nov 252012 Nov 30

Publication series

NameMaterials Research Society Symposium Proceedings
ISSN (Print)0272-9172


Conference2012 MRS Fall Meeting
Country/TerritoryUnited States
CityBoston, MA


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