TY - JOUR
T1 - Investigation of colony disruption for hydrocarbon extraction from Botryococcus braunii
AU - Tsutsumi, Shun
AU - Saito, Yasuhiro
AU - Matsushita, Yohsuke
AU - Aoki, Hideyuki
N1 - Funding Information:
This work was supported by Next-generation Energies for Tohoku Recovery (NET) project of the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan. The samples of B. braunii were provided by University of Tsukuba .
Funding Information:
This work was supported by Next-generation Energies for Tohoku Recovery (NET) project of the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan. The samples of B. braunii were provided by University of Tsukuba.
Publisher Copyright:
© 2017 Elsevier B.V.
PY - 2018/4
Y1 - 2018/4
N2 - Botryococcus braunii is a colonial microalga that produces hydrocarbons. While the microalga stores almost all the amount of hydrocarbons in its colony matrix, the amount of the extracted hydrocarbon without any pretreatment is typically very small. We performed mechanical cell disruption using a JET PASTER® and a bead mill as the pretreatment ways to facilitate hydrocarbon extraction from B. braunii. After the disruption, the size and shape of colonies changed. In the JET PASTER treatment at 4800 rpm, the concentration of removed polysaccharides increased 146 to 173 μg/mL and the hydrocarbon yield increased 2.7 to 82.8%. In the bead mill treatment, the concentration of removed polysaccharides increased 146 to 210 μg/mL and hydrocarbon yield increased 2.7 to 42.3%. Therefore, the disruption of colonies and polysaccharides around algal colonies would affect the hydrocarbon extraction. In addition, the apparent photosynthetic activity of the sample treated by the JET PASTER was 0.71 that is almost the same value as that of the untreated sample, whereas that of the sample treated by the bead mill was 0.64. Therefore, the JET PASTER treatment did not affect the photosynthetic function of B. braunii.
AB - Botryococcus braunii is a colonial microalga that produces hydrocarbons. While the microalga stores almost all the amount of hydrocarbons in its colony matrix, the amount of the extracted hydrocarbon without any pretreatment is typically very small. We performed mechanical cell disruption using a JET PASTER® and a bead mill as the pretreatment ways to facilitate hydrocarbon extraction from B. braunii. After the disruption, the size and shape of colonies changed. In the JET PASTER treatment at 4800 rpm, the concentration of removed polysaccharides increased 146 to 173 μg/mL and the hydrocarbon yield increased 2.7 to 82.8%. In the bead mill treatment, the concentration of removed polysaccharides increased 146 to 210 μg/mL and hydrocarbon yield increased 2.7 to 42.3%. Therefore, the disruption of colonies and polysaccharides around algal colonies would affect the hydrocarbon extraction. In addition, the apparent photosynthetic activity of the sample treated by the JET PASTER was 0.71 that is almost the same value as that of the untreated sample, whereas that of the sample treated by the bead mill was 0.64. Therefore, the JET PASTER treatment did not affect the photosynthetic function of B. braunii.
KW - Botryococcus braunii
KW - Colony disruption
KW - Colony shape
KW - Hydrocarbon extraction
KW - Mechanical cell disruption
KW - Photosynthetic activity
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U2 - 10.1016/j.fuproc.2017.12.004
DO - 10.1016/j.fuproc.2017.12.004
M3 - Article
AN - SCOPUS:85037711968
SN - 0378-3820
VL - 172
SP - 36
EP - 48
JO - Fuel Processing Technology
JF - Fuel Processing Technology
ER -