TY - JOUR
T1 - Involvement of H+-gradient dependent transporter in PGE2 release from A549 cells
AU - Tanaka, Nobuaki
AU - Yamaguchi, Hiroaki
AU - Mano, Nariyasu
N1 - Funding Information:
This work was in part supported by Japan Society for the Promotion of Science (JSPS) KAKENHI grant number 25460184 and Towa Foundation for Food Science and Research . We would like to thank Editage ( www.editage.jp ) for English language editing.
Publisher Copyright:
© 2019 Elsevier Ltd
PY - 2019/10
Y1 - 2019/10
N2 - The purpose of this study was to identify the transporter involved in the release of prostaglandin E2 (PGE2). In the present study, transport assays were conducted using membrane vesicles prepared from human lung adenocarcinoma A549 cells, thus enabling identification of the novel exporter present in A549 cells. PGE2 transport into A549 vesicles was higher in the presence of a proton (H+)-gradient, thus suggesting the involvement of PGE2–H+ symporter in PGE2 transport. Results from our experiments showed enhanced PGE2 release in A549 cells in the presence of H+-gradient ([H+]extracellular < [H+]intracellular). Moreover, in vesicular transport assays, H+-gradient-dependent transport of PGE2 did not show saturation up to 500 μM PGE2, and 10 mM aromatic monocarboxylic acids (acetylsalicylic acid, salicylic acid, and p-nitrobenzoic acid) significantly inhibited PGE2 transport by 62–70%. These results suggest, the involvement of monocarboxylate transporters in the H+-gradient-dependent PGE2 export.
AB - The purpose of this study was to identify the transporter involved in the release of prostaglandin E2 (PGE2). In the present study, transport assays were conducted using membrane vesicles prepared from human lung adenocarcinoma A549 cells, thus enabling identification of the novel exporter present in A549 cells. PGE2 transport into A549 vesicles was higher in the presence of a proton (H+)-gradient, thus suggesting the involvement of PGE2–H+ symporter in PGE2 transport. Results from our experiments showed enhanced PGE2 release in A549 cells in the presence of H+-gradient ([H+]extracellular < [H+]intracellular). Moreover, in vesicular transport assays, H+-gradient-dependent transport of PGE2 did not show saturation up to 500 μM PGE2, and 10 mM aromatic monocarboxylic acids (acetylsalicylic acid, salicylic acid, and p-nitrobenzoic acid) significantly inhibited PGE2 transport by 62–70%. These results suggest, the involvement of monocarboxylate transporters in the H+-gradient-dependent PGE2 export.
KW - Mass spectrometry
KW - Monocarboxylate transporters
KW - Prostaglandin
KW - Release, H-gradient
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U2 - 10.1016/j.plefa.2019.08.003
DO - 10.1016/j.plefa.2019.08.003
M3 - Article
C2 - 31421525
AN - SCOPUS:85070537369
SN - 0952-3278
VL - 149
SP - 30
EP - 36
JO - Prostaglandins Leukotrienes and Essential Fatty Acids
JF - Prostaglandins Leukotrienes and Essential Fatty Acids
ER -