Involvement of Rac GTPase activation in phosphatidylcholine hydroperoxide-induced THP-1 cell adhesion to ICAM-1

Akira Asai; Fumitaka Okajima; Yasushi Nakajima; Mototsugu Nagao; Kiyotaka Nakagawa; Teruo Miyazawa; Shinichi Oikawa

doi:10.1016/j.bbrc.2011.02.032

Involvement of Rac GTPase activation in phosphatidylcholine hydroperoxide-induced THP-1 cell adhesion to ICAM-1

Akira Asai, Fumitaka Okajima, Yasushi Nakajima, Mototsugu Nagao, Kiyotaka Nakagawa, Teruo Miyazawa, Shinichi Oikawa

New Industry Creation Hatchery Center (NICHe)

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

Increasing evidence indicates that phospholipid oxidation plays important roles in atherosclerosis. Here, we investigated the involvement of Rho-family GTPases inphosphatidylcholine hydroperoxide (PCOOH)-induced THP-1 cell adhesion to ICAM-1. Isoprenoid depletion by fluvastatin and geranylgeranyltransferase inhibition by GGTI-286 suppressed PCOOH-induced cell adhesion to ICAM-1 and F-actin-rich membrane protrusion formation. Pull-down assays demonstrated the activation of Rac1 and Rac2 in PCOOH-treated cells. Pan-Rho-family GTPase inhibitor Clostridium difficile toxin B, Rac-specific inhibitor NSC23776, and RNA interference of the Rac isoforms suppressed the cell adhesion. These findings indicate the involvement of Rac GTPase activation in PCOOH-induced cell adhesion to ICAM-1 via actin reorganization.

Original language	English
Pages (from-to)	273-277
Number of pages	5
Journal	Biochemical and biophysical research communications
Volume	406
Issue number	2
DOIs	https://doi.org/10.1016/j.bbrc.2011.02.032
Publication status	Published - 2011 Mar 11

Keywords

Actin
Cell adhesion
Monocyte
Phosphatidylcholine hydroperoxide
Phospholipid oxidation
Rac

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2011.02.032

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title = "Involvement of Rac GTPase activation in phosphatidylcholine hydroperoxide-induced THP-1 cell adhesion to ICAM-1",

abstract = "Increasing evidence indicates that phospholipid oxidation plays important roles in atherosclerosis. Here, we investigated the involvement of Rho-family GTPases inphosphatidylcholine hydroperoxide (PCOOH)-induced THP-1 cell adhesion to ICAM-1. Isoprenoid depletion by fluvastatin and geranylgeranyltransferase inhibition by GGTI-286 suppressed PCOOH-induced cell adhesion to ICAM-1 and F-actin-rich membrane protrusion formation. Pull-down assays demonstrated the activation of Rac1 and Rac2 in PCOOH-treated cells. Pan-Rho-family GTPase inhibitor Clostridium difficile toxin B, Rac-specific inhibitor NSC23776, and RNA interference of the Rac isoforms suppressed the cell adhesion. These findings indicate the involvement of Rac GTPase activation in PCOOH-induced cell adhesion to ICAM-1 via actin reorganization.",

keywords = "Actin, Cell adhesion, Monocyte, Phosphatidylcholine hydroperoxide, Phospholipid oxidation, Rac",

author = "Akira Asai and Fumitaka Okajima and Yasushi Nakajima and Mototsugu Nagao and Kiyotaka Nakagawa and Teruo Miyazawa and Shinichi Oikawa",

note = "Funding Information: The authors thank Momoyo Kawahara for excellent technical assistance. This study was supported in part by a Grants-in-Aid for Scientific Research (C) to S.O. (21591165) and (S) to T.M. (20228002) from the Japan Society for the Promotion of Science.",

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doi = "10.1016/j.bbrc.2011.02.032",

language = "English",

volume = "406",

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journal = "Biochemical and Biophysical Research Communications",

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T1 - Involvement of Rac GTPase activation in phosphatidylcholine hydroperoxide-induced THP-1 cell adhesion to ICAM-1

AU - Asai, Akira

AU - Okajima, Fumitaka

AU - Nakajima, Yasushi

AU - Nagao, Mototsugu

AU - Nakagawa, Kiyotaka

AU - Miyazawa, Teruo

AU - Oikawa, Shinichi

N1 - Funding Information: The authors thank Momoyo Kawahara for excellent technical assistance. This study was supported in part by a Grants-in-Aid for Scientific Research (C) to S.O. (21591165) and (S) to T.M. (20228002) from the Japan Society for the Promotion of Science.

PY - 2011/3/11

Y1 - 2011/3/11

N2 - Increasing evidence indicates that phospholipid oxidation plays important roles in atherosclerosis. Here, we investigated the involvement of Rho-family GTPases inphosphatidylcholine hydroperoxide (PCOOH)-induced THP-1 cell adhesion to ICAM-1. Isoprenoid depletion by fluvastatin and geranylgeranyltransferase inhibition by GGTI-286 suppressed PCOOH-induced cell adhesion to ICAM-1 and F-actin-rich membrane protrusion formation. Pull-down assays demonstrated the activation of Rac1 and Rac2 in PCOOH-treated cells. Pan-Rho-family GTPase inhibitor Clostridium difficile toxin B, Rac-specific inhibitor NSC23776, and RNA interference of the Rac isoforms suppressed the cell adhesion. These findings indicate the involvement of Rac GTPase activation in PCOOH-induced cell adhesion to ICAM-1 via actin reorganization.

AB - Increasing evidence indicates that phospholipid oxidation plays important roles in atherosclerosis. Here, we investigated the involvement of Rho-family GTPases inphosphatidylcholine hydroperoxide (PCOOH)-induced THP-1 cell adhesion to ICAM-1. Isoprenoid depletion by fluvastatin and geranylgeranyltransferase inhibition by GGTI-286 suppressed PCOOH-induced cell adhesion to ICAM-1 and F-actin-rich membrane protrusion formation. Pull-down assays demonstrated the activation of Rac1 and Rac2 in PCOOH-treated cells. Pan-Rho-family GTPase inhibitor Clostridium difficile toxin B, Rac-specific inhibitor NSC23776, and RNA interference of the Rac isoforms suppressed the cell adhesion. These findings indicate the involvement of Rac GTPase activation in PCOOH-induced cell adhesion to ICAM-1 via actin reorganization.

KW - Actin

KW - Cell adhesion

KW - Monocyte

KW - Phosphatidylcholine hydroperoxide

KW - Phospholipid oxidation

KW - Rac

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Involvement of Rac GTPase activation in phosphatidylcholine hydroperoxide-induced THP-1 cell adhesion to ICAM-1

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