TY - JOUR
T1 - Involvement of the elicitor-induced gene OsWRKY53 in the expression of defense-related genes in rice
AU - Chujo, Tetsuya
AU - Takai, Ryota
AU - Akimoto-Tomiyama, Chiharu
AU - Ando, Sugihiro
AU - Minami, Eiichi
AU - Nagamura, Yoshiaki
AU - Kaku, Hanae
AU - Shibuya, Naoto
AU - Yasuda, Michiko
AU - Nakashita, Hideo
AU - Umemura, Kenji
AU - Okada, Atsushi
AU - Okada, Kazunori
AU - Nojiri, Hideaki
AU - Yamane, Hisakazu
N1 - Funding Information:
We thank Drs. T. Kawasaki and K. Shimamoto (Nara Institute of Science and Technology) and Dr. J. Kyozuka (Graduate School of Agriculture and Life Science, University of Tokyo) for the gift of pSN221, Dr. T. Nakagawa (Shimane University) for the gift of pGWB2 and pGWB6, and Ms. R. Motoyama for technical assistance in microarray analysis. This work was supported in part by Grants-in-Aid for Scientific Research (No. 12460051 and No. 15380080) to H. Y. from the Japanese Society for the Promotion of Science, and by the Program for Promotion of Basic Research Activities for Innovative Biosciences (PROBRAIN).
PY - 2007/7
Y1 - 2007/7
N2 - We present a detailed characterization of the chitin oligosaccharide elicitor-induced gene OsWRKY53. OsWRKY53 was also induced in suspension-cultured rice cells by a fungal cerebroside elicitor and in rice plants by infection with the blast fungus Magnaporthe grisea. A fusion of OsWRKY53 with green fluorescent protein was detected exclusively in the nuclei of onion epidermal cells, and OsWRKY53 protein specifically bound to W-box elements. A transient assay using the particle bombardment method showed that OsWRKY53 is a transcriptional activator. A microarray analysis revealed that several defense-related genes, including pathogenesis-related protein genes such as PBZ1, were upregulated in rice cells overexpressing OsWRKY53. Finally, overexpression of OsWRKY53 in rice plants resulted in enhanced resistance to M. grisea. These results strongly suggest that OsWRKY53 is a transcription factor that plays important roles in elicitor-induced defense signaling pathways in rice.
AB - We present a detailed characterization of the chitin oligosaccharide elicitor-induced gene OsWRKY53. OsWRKY53 was also induced in suspension-cultured rice cells by a fungal cerebroside elicitor and in rice plants by infection with the blast fungus Magnaporthe grisea. A fusion of OsWRKY53 with green fluorescent protein was detected exclusively in the nuclei of onion epidermal cells, and OsWRKY53 protein specifically bound to W-box elements. A transient assay using the particle bombardment method showed that OsWRKY53 is a transcriptional activator. A microarray analysis revealed that several defense-related genes, including pathogenesis-related protein genes such as PBZ1, were upregulated in rice cells overexpressing OsWRKY53. Finally, overexpression of OsWRKY53 in rice plants resulted in enhanced resistance to M. grisea. These results strongly suggest that OsWRKY53 is a transcription factor that plays important roles in elicitor-induced defense signaling pathways in rice.
KW - Defense response
KW - Elicitor
KW - Oryza sativa L. cv. Nipponbare
KW - WRKY
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U2 - 10.1016/j.bbaexp.2007.04.006
DO - 10.1016/j.bbaexp.2007.04.006
M3 - Article
C2 - 17532485
AN - SCOPUS:34447538086
SN - 0167-4781
VL - 1769
SP - 497
EP - 505
JO - Biochimica et Biophysica Acta - Gene Structure and Expression
JF - Biochimica et Biophysica Acta - Gene Structure and Expression
IS - 7-8
ER -