We isolated 11 low-indole-3-acetic acid (IAA)-producing mutants of Bradyrhizobium elkanii by Tn5 mutagenesis. The amount of IAA produced by each mutant was 2.2-13.6% of that of the wild-type. It was found by resting cell reactions that the biosynthetic step to convert indole-3-pyruvic acid to indole-3-acetaldehyde was blocked in all the mutants.
- Biosynthesis of indole-3-acetic acid
- Bradyrhizobium elkanii
- Indole-3-pyruvic acid decarboxylase
- Tn5 mutagenesis