Isolation and characterization of low-indole-3-acetic acid-producing mutants from bradyrhizobium elkanii

Ken Yagi; Taku Matsumoto; Tetsuya Chujo; Hideaki Nojiri; Toshio Omori; Kiwamu Minamisawa; Makoto Nishiyama; Hisakazu Yamane

doi:10.1271/bbb.64.1359

Isolation and characterization of low-indole-3-acetic acid-producing mutants from bradyrhizobium elkanii

Ken Yagi, Taku Matsumoto, Tetsuya Chujo, Hideaki Nojiri, Toshio Omori, Kiwamu Minamisawa, Makoto Nishiyama, Hisakazu Yamane

LIF - Ecological Developmental Adaptability Life Sciences

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

We isolated 11 low-indole-3-acetic acid (IAA)-producing mutants of Bradyrhizobium elkanii by Tn5 mutagenesis. The amount of IAA produced by each mutant was 2.2-13.6% of that of the wild-type. It was found by resting cell reactions that the biosynthetic step to convert indole-3-pyruvic acid to indole-3-acetaldehyde was blocked in all the mutants.

Original language	English
Pages (from-to)	1359-1364
Number of pages	6
Journal	Bioscience, Biotechnology and Biochemistry
Volume	64
Issue number	7
DOIs	https://doi.org/10.1271/bbb.64.1359
Publication status	Published - 2000

Keywords

Biosynthesis of indole-3-acetic acid
Bradyrhizobium elkanii
Indole-3-pyruvic acid decarboxylase
Tn5 mutagenesis

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title = "Isolation and characterization of low-indole-3-acetic acid-producing mutants from bradyrhizobium elkanii",

abstract = "We isolated 11 low-indole-3-acetic acid (IAA)-producing mutants of Bradyrhizobium elkanii by Tn5 mutagenesis. The amount of IAA produced by each mutant was 2.2-13.6% of that of the wild-type. It was found by resting cell reactions that the biosynthetic step to convert indole-3-pyruvic acid to indole-3-acetaldehyde was blocked in all the mutants.",

keywords = "Biosynthesis of indole-3-acetic acid, Bradyrhizobium elkanii, Indole-3-pyruvic acid decarboxylase, Tn5 mutagenesis",

author = "Ken Yagi and Taku Matsumoto and Tetsuya Chujo and Hideaki Nojiri and Toshio Omori and Kiwamu Minamisawa and Makoto Nishiyama and Hisakazu Yamane",

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doi = "10.1271/bbb.64.1359",

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TY - JOUR

T1 - Isolation and characterization of low-indole-3-acetic acid-producing mutants from bradyrhizobium elkanii

AU - Yagi, Ken

AU - Matsumoto, Taku

AU - Chujo, Tetsuya

AU - Nojiri, Hideaki

AU - Omori, Toshio

AU - Minamisawa, Kiwamu

AU - Nishiyama, Makoto

AU - Yamane, Hisakazu

PY - 2000

Y1 - 2000

N2 - We isolated 11 low-indole-3-acetic acid (IAA)-producing mutants of Bradyrhizobium elkanii by Tn5 mutagenesis. The amount of IAA produced by each mutant was 2.2-13.6% of that of the wild-type. It was found by resting cell reactions that the biosynthetic step to convert indole-3-pyruvic acid to indole-3-acetaldehyde was blocked in all the mutants.

AB - We isolated 11 low-indole-3-acetic acid (IAA)-producing mutants of Bradyrhizobium elkanii by Tn5 mutagenesis. The amount of IAA produced by each mutant was 2.2-13.6% of that of the wild-type. It was found by resting cell reactions that the biosynthetic step to convert indole-3-pyruvic acid to indole-3-acetaldehyde was blocked in all the mutants.

KW - Biosynthesis of indole-3-acetic acid

KW - Bradyrhizobium elkanii

KW - Indole-3-pyruvic acid decarboxylase

KW - Tn5 mutagenesis

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AN - SCOPUS:0034220796

SN - 0916-8451

VL - 64

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JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

IS - 7

ER -

Isolation and characterization of low-indole-3-acetic acid-producing mutants from bradyrhizobium elkanii

Abstract

Keywords

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