Isolation and characterization of microsatellite DNA loci for endangered fish, Japanese huchen (Hucho perryi)

Makoto Hatakeyama; Tomohisa Watanabe; Minoru Ikeda; Masamichi Nakajima; Hiroshi Kawamula; Nobuhiko Taniguchi

doi:10.1111/j.1471-8286.2005.01101.x

Isolation and characterization of microsatellite DNA loci for endangered fish, Japanese huchen (Hucho perryi)

Makoto Hatakeyama, Tomohisa Watanabe, Minoru Ikeda, Masamichi Nakajima, Hiroshi Kawamula, Nobuhiko Taniguchi

AGR - Field Science Center

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Japanese huchen, Hucho perryi, is an endangered fish in Japan. In order to promote a conservation program for this species, it is necessary to evaluate its genetic diversity and population genetics. For this purpose, we designed 15 primers containing dinucleotide microsatellite regions and examined their allelic variation using a total of 23 wild individuals collected from two different localities. Eight loci showed moderate allelic variation ranging from two to four alleles, with expected heterozygosities from 0.043 to 0.548. These markers will be useful for the assessment of genetic variation for this species.

Original language	English
Pages (from-to)	893-895
Number of pages	3
Journal	Molecular Ecology Notes
Volume	5
Issue number	4
DOIs	https://doi.org/10.1111/j.1471-8286.2005.01101.x
Publication status	Published - 2005 Dec 1

Keywords

Endangered fish
Hucho perryi
Japanese huchen
Microsatellite DNA

ASJC Scopus subject areas

Biochemistry
Ecology
Biochemistry, Genetics and Molecular Biology(all)

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10.1111/j.1471-8286.2005.01101.x

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abstract = "Japanese huchen, Hucho perryi, is an endangered fish in Japan. In order to promote a conservation program for this species, it is necessary to evaluate its genetic diversity and population genetics. For this purpose, we designed 15 primers containing dinucleotide microsatellite regions and examined their allelic variation using a total of 23 wild individuals collected from two different localities. Eight loci showed moderate allelic variation ranging from two to four alleles, with expected heterozygosities from 0.043 to 0.548. These markers will be useful for the assessment of genetic variation for this species.",

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T1 - Isolation and characterization of microsatellite DNA loci for endangered fish, Japanese huchen (Hucho perryi)

AU - Hatakeyama, Makoto

AU - Watanabe, Tomohisa

AU - Ikeda, Minoru

AU - Nakajima, Masamichi

AU - Kawamula, Hiroshi

AU - Taniguchi, Nobuhiko

PY - 2005/12/1

Y1 - 2005/12/1

N2 - Japanese huchen, Hucho perryi, is an endangered fish in Japan. In order to promote a conservation program for this species, it is necessary to evaluate its genetic diversity and population genetics. For this purpose, we designed 15 primers containing dinucleotide microsatellite regions and examined their allelic variation using a total of 23 wild individuals collected from two different localities. Eight loci showed moderate allelic variation ranging from two to four alleles, with expected heterozygosities from 0.043 to 0.548. These markers will be useful for the assessment of genetic variation for this species.

AB - Japanese huchen, Hucho perryi, is an endangered fish in Japan. In order to promote a conservation program for this species, it is necessary to evaluate its genetic diversity and population genetics. For this purpose, we designed 15 primers containing dinucleotide microsatellite regions and examined their allelic variation using a total of 23 wild individuals collected from two different localities. Eight loci showed moderate allelic variation ranging from two to four alleles, with expected heterozygosities from 0.043 to 0.548. These markers will be useful for the assessment of genetic variation for this species.

KW - Endangered fish

KW - Hucho perryi

KW - Japanese huchen

KW - Microsatellite DNA

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Isolation and characterization of microsatellite DNA loci for endangered fish, Japanese huchen (Hucho perryi)

Abstract

Keywords

ASJC Scopus subject areas

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