Isolation and characterization of synovial cells from the human temporomandibular joint

H. Nagai, Y. Miyamoto, A. Nakata, S. Hatakeyama, Y. Iwanami, M. Fukuda

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)


BACKGROUND: The synovial tissues with temporomandibular disorders (TMDs) often show chronic inflammatory changes and the synovial cells participate in the pathogenic processes of TMDs. The synovial membrane is composed of a synovial lining layer and a connective sublining layer. The synovial lining layer is made up of two kinds of cells: macrophage-like type A and fibroblastic type B cells. The aim of this study was to isolate and characterize synovial cells from the human temporomandibular joint (TMJ). METHODS: Synovial cells were isolated using an explant culture method. Then, we characterized the cultured synovial cells (SGA2 cells) using immunocytochemistry. RESULTS: SGA2 cells expressed the fibroblastic markers vimentin and prolyl 4-hydroxylase; they also expressed laminin and heat shock protein 27, all of which are markers of type B cells. However, some cells expressed the macrophage marker CD68. These CD68-positive cells simultaneously expressed laminin. CONCLUSIONS: We isolated and cultured synovial type B cells from the human TMJ, and identified the presence of intermediate type synovial lining cells, having the phenotypic properties of both type A and type B cells, among the synovial lining cells.

Original languageEnglish
Pages (from-to)104-110
Number of pages7
JournalJournal of Oral Pathology and Medicine
Issue number2
Publication statusPublished - 2006 Feb
Externally publishedYes


  • Synovial membrane
  • Temporomandibular disorders
  • Temporomandibular joint

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Oral Surgery
  • Otorhinolaryngology
  • Cancer Research
  • Periodontics


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