Isolation and structural organization of the human preproenkephalin Bgene

Saburo Horikawa, Toshiyuki Takai, Mitsuyoshi Toyosato, Hideo Takahashi, Masaharu Noda, Hitoshi Kakidani, Tai Kubo, Tadaaki Hirose, Seiichi Inayama, Hidenori Hayashida, Takashi Miyata, Shosaku Numa

Research output: Contribution to journalArticlepeer-review

178 Citations (Scopus)


The primary structure of porcine preproenkephalin B has been elucidated by cloning and sequencing cDNA1: it contains neoendorphin2,3, dynorphin4,5 and leumorphin1,6,7 (containing rimorphin as its ammo-terminus)8,9. These opioid peptides, each having a leucine-enkephalin structure10, act on the κ-receptor 7,11-14. We have now cloned a human genomic DNA segment containing the preproenkephalin B gene. The structural organization of this gene resembles those of the genes encoding the other opioid peptide precursors, that is, preproenkephalin A15 and the corticotropin-β-lipotropin precursor16-21 (ACTH-β-LPH precursor). The primary structure of human preproenkephalin B has been deduced from the gene sequence. The amino acid sequence homology observed between preproenkephalin B and preproenkephalin A, together with the similarity between their gene organizations, suggests that the two genes have been generated from a common ancestor by gene duplication.

Original languageEnglish
Pages (from-to)611-614
Number of pages4
Issue number5943
Publication statusPublished - 1983


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