TY - JOUR
T1 - Isolation of a New Minor Protein (Ovofactor-1), Which Has a Cell Growth Promoting Activity, from Hen's Egg White by Heparin Affinity Chromatography
AU - Nakamura, Tadashi
AU - Saito, Tadao
AU - Kitazawa, Haruki
AU - Takeuchi, Sanae
AU - Itoh, Takatoshi
PY - 1995
Y1 - 1995
N2 - A proteinaceous component eluted at the concentration of 0.8-1.0 M NaCl was obtained from hen egg white by heparin affinity chromatography. On SDS-PAGE, the component was resolved into three bands of 18. 5, 16. 5, and 16.0 kDa. The isoelectric point was estimated to be pH 8.8. This stimulated the DNA synthesis and proliferation of the cultured cells from chichen embryos. The amino acid composition and N-terminal analyses of the main 18.5 kDa component showed that this is a novel minor protein in egg white, and therefore was named “Ovofactor-1”.
AB - A proteinaceous component eluted at the concentration of 0.8-1.0 M NaCl was obtained from hen egg white by heparin affinity chromatography. On SDS-PAGE, the component was resolved into three bands of 18. 5, 16. 5, and 16.0 kDa. The isoelectric point was estimated to be pH 8.8. This stimulated the DNA synthesis and proliferation of the cultured cells from chichen embryos. The amino acid composition and N-terminal analyses of the main 18.5 kDa component showed that this is a novel minor protein in egg white, and therefore was named “Ovofactor-1”.
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U2 - 10.1271/bbb.59.1946
DO - 10.1271/bbb.59.1946
M3 - Article
C2 - 8534988
AN - SCOPUS:0029385206
SN - 0916-8451
VL - 59
SP - 1946
EP - 1948
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
IS - 10
ER -