Isolation of adult human pluripotent stem cells from mesenchymal cell populations and their application to liver damages

Shohei Wakao; Masaaki Kitada; Yasumasa Kuroda; Mari Dezawa

doi:10.1007/978-1-61779-468-1_8

Isolation of adult human pluripotent stem cells from mesenchymal cell populations and their application to liver damages

Shohei Wakao, Masaaki Kitada, Yasumasa Kuroda, Mari Dezawa

MED - Medical Sciences

Tohoku University

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

17 Citations (Scopus)

Abstract

We have found a novel type of pluripotent stem cells, Multilineage- differentiating stress enduring (Muse) cells that can be isolated from mesenchymal cell populations. Muse cells are characterized by stress tolerance, expression of pluripotency markers, self-renewal, and the ability to differentiate into endodermal-, mesodermal-, and ectodermal-lineage cells from a single cell, demonstrating that they are pluripotent stem cells. They can be isolated as cells positive for stage-specific embryonic antigen-3, a human pluripotent stem cell marker. Here, we introduce the isolation method for Muse cells and the effect of transplantation of these cells on chronic liver diseases.

Original language	English
Title of host publication	Liver Stem Cells
Subtitle of host publication	Methods and Protocols
Editors	Ochiya Takahiro
Pages	89-102
Number of pages	14
DOIs	https://doi.org/10.1007/978-1-61779-468-1_8
Publication status	Published - 2012

Publication series

Name	Methods in Molecular Biology
Volume	826
ISSN (Print)	1064-3745

Keywords

Chronic liver diseases
Muse
Pluripotent stem cells
SSEA-3
Transplantation

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/978-1-61779-468-1_8

Cite this

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abstract = "We have found a novel type of pluripotent stem cells, Multilineage- differentiating stress enduring (Muse) cells that can be isolated from mesenchymal cell populations. Muse cells are characterized by stress tolerance, expression of pluripotency markers, self-renewal, and the ability to differentiate into endodermal-, mesodermal-, and ectodermal-lineage cells from a single cell, demonstrating that they are pluripotent stem cells. They can be isolated as cells positive for stage-specific embryonic antigen-3, a human pluripotent stem cell marker. Here, we introduce the isolation method for Muse cells and the effect of transplantation of these cells on chronic liver diseases.",

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Isolation of adult human pluripotent stem cells from mesenchymal cell populations and their application to liver damages. / Wakao, Shohei; Kitada, Masaaki; Kuroda, Yasumasa et al.
Liver Stem Cells: Methods and Protocols. ed. / Ochiya Takahiro. 2012. p. 89-102 (Methods in Molecular Biology; Vol. 826).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

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T1 - Isolation of adult human pluripotent stem cells from mesenchymal cell populations and their application to liver damages

AU - Wakao, Shohei

AU - Kitada, Masaaki

AU - Kuroda, Yasumasa

AU - Dezawa, Mari

PY - 2012

Y1 - 2012

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KW - Chronic liver diseases

KW - Muse

KW - Pluripotent stem cells

KW - SSEA-3

KW - Transplantation

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Isolation of adult human pluripotent stem cells from mesenchymal cell populations and their application to liver damages

Abstract

Publication series

Keywords

UN SDGs

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