TY - JOUR
T1 - Isolation of DNA sequences amplified at chromosome 19q13.1-q13.2 including the AKT2 locus in human pancreatic cancer
AU - Miwa, Wataru
AU - Yasuda, Jun
AU - Murakami, Yoshinori
AU - Yashima, Kazuo
AU - Sugano, Kokichi
AU - Sekine, Teruaki
AU - Kono, Akira
AU - Egawa, Shinichi
AU - Yamaguchi, Ken
AU - Hayashizaki, Yoshihide
AU - Sekiya, Takao
N1 - Funding Information:
1This work was supported in part by a Grant-in-Aid from the Ministry of Health and Welfare for the 2nd Term Comprehensive 10-Year Strategy for Cancer Control, Japan, a Research Grant on Aging and Health from the Ministry of Health and Welfare, a grant from the Special Coordination Fund of Science and Technology Agency of Japan. W. M. is a recipient of a Research Resident Fellowship from the Foundation for Promotion of Cancer Research of Japan. 2To whom correspondence should be addressed. Fax: /81–5565–9535. E-mail: tsekiya@ncc.go.jp.
PY - 1996/8/23
Y1 - 1996/8/23
N2 - In the human pancreatic cancer cell Line PANC1, we detected several DNA fragments with abnormally intensified signals by restriction landmark genomic scanning. Major five of these fragments were cloned. All of the cloned fragments were mapped at the 19q13.1-13.2 region where the AKT2 oncogene was located. Southern blotting using the cloned DNA fragments and a fragment of AKT2 cDNA as probes revealed that the AKT2 gene was amplified in 3 of 12 pancreatic cancer cell lines analyzed including PANC1 and in 3 of 20 primary pancreatic cancers. The AKT2 gene was overexpressed in the 3 cell lines with the amplified gene. The results suggest that the AKT2 gene is a candidate oncogene activated by amplification in some human pancreatic cancers.
AB - In the human pancreatic cancer cell Line PANC1, we detected several DNA fragments with abnormally intensified signals by restriction landmark genomic scanning. Major five of these fragments were cloned. All of the cloned fragments were mapped at the 19q13.1-13.2 region where the AKT2 oncogene was located. Southern blotting using the cloned DNA fragments and a fragment of AKT2 cDNA as probes revealed that the AKT2 gene was amplified in 3 of 12 pancreatic cancer cell lines analyzed including PANC1 and in 3 of 20 primary pancreatic cancers. The AKT2 gene was overexpressed in the 3 cell lines with the amplified gene. The results suggest that the AKT2 gene is a candidate oncogene activated by amplification in some human pancreatic cancers.
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U2 - 10.1006/bbrc.1996.1280
DO - 10.1006/bbrc.1996.1280
M3 - Article
C2 - 8780719
AN - SCOPUS:0030598884
SN - 0006-291X
VL - 225
SP - 968
EP - 974
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -
