Isolation of genes abundantly expressed in rice anthers at the microspore stage

Tohru Tsuchiya; Kinya Toriyama; Mikhail E. Nasrallah; Shin ichiro Ejiri

doi:10.1007/BF00028907

Isolation of genes abundantly expressed in rice anthers at the microspore stage

Tohru Tsuchiya, Kinya Toriyama, Mikhail E. Nasrallah, Shin ichiro Ejiri

Research output: Contribution to journal › Article › peer-review

33 Citations (Scopus)

Abstract

A cDNA library of rice (Oryza sativa L.) has been constructed from anthers at an early stage of pollen development. By differential screening of the library, we have isolated cDNAs of two genes, designated as Osc4 and Osc6, that are abundantly expressed in anthers containing tetrads and uninucleate microspores, but are not expressed in leaves or roots. Expression of Osc4 is absent in mature anthers, while Osc6 is present although the expression decays during pollen maturation. A comparison of the nucleotide and deduced amino acid sequences with those in data banks has not shown significant homology to known molecules.

Original language	English
Pages (from-to)	1189-1193
Number of pages	5
Journal	Plant Molecular Biology
Volume	20
Issue number	6
DOIs	https://doi.org/10.1007/BF00028907
Publication status	Published - 1992 Dec 1
Externally published	Yes

Keywords

anther development
cDNA
rice

ASJC Scopus subject areas

Agronomy and Crop Science
Genetics
Plant Science

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10.1007/BF00028907

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abstract = "A cDNA library of rice (Oryza sativa L.) has been constructed from anthers at an early stage of pollen development. By differential screening of the library, we have isolated cDNAs of two genes, designated as Osc4 and Osc6, that are abundantly expressed in anthers containing tetrads and uninucleate microspores, but are not expressed in leaves or roots. Expression of Osc4 is absent in mature anthers, while Osc6 is present although the expression decays during pollen maturation. A comparison of the nucleotide and deduced amino acid sequences with those in data banks has not shown significant homology to known molecules.",

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T1 - Isolation of genes abundantly expressed in rice anthers at the microspore stage

AU - Tsuchiya, Tohru

AU - Toriyama, Kinya

AU - Nasrallah, Mikhail E.

AU - Ejiri, Shin ichiro

PY - 1992/12/1

Y1 - 1992/12/1

N2 - A cDNA library of rice (Oryza sativa L.) has been constructed from anthers at an early stage of pollen development. By differential screening of the library, we have isolated cDNAs of two genes, designated as Osc4 and Osc6, that are abundantly expressed in anthers containing tetrads and uninucleate microspores, but are not expressed in leaves or roots. Expression of Osc4 is absent in mature anthers, while Osc6 is present although the expression decays during pollen maturation. A comparison of the nucleotide and deduced amino acid sequences with those in data banks has not shown significant homology to known molecules.

AB - A cDNA library of rice (Oryza sativa L.) has been constructed from anthers at an early stage of pollen development. By differential screening of the library, we have isolated cDNAs of two genes, designated as Osc4 and Osc6, that are abundantly expressed in anthers containing tetrads and uninucleate microspores, but are not expressed in leaves or roots. Expression of Osc4 is absent in mature anthers, while Osc6 is present although the expression decays during pollen maturation. A comparison of the nucleotide and deduced amino acid sequences with those in data banks has not shown significant homology to known molecules.

KW - anther development

KW - cDNA

KW - rice

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Isolation of genes abundantly expressed in rice anthers at the microspore stage

Abstract

Keywords

ASJC Scopus subject areas

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