Isoproterenol stimulates transient SNAP23-VAMP2 interaction in rat parotid glands

Taishin Takuma; Akiko Shitara; Toshiya Arakawa; Miki Okayama; Itaru Mizoguchi; Yoshifumi Tajima

doi:10.1016/j.febslet.2013.01.039

Isoproterenol stimulates transient SNAP23-VAMP2 interaction in rat parotid glands

Taishin Takuma, Akiko Shitara, Toshiya Arakawa, Miki Okayama, Itaru Mizoguchi, Yoshifumi Tajima

DEN - Dental Sciences

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

The exocytosis of salivary proteins is mainly regulated by cAMP, although soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs), which mediate cAMP-dependent exocytic membrane fusion, have remained unidentified. Here we examined the effect of isoproterenol (ISO) and cytochalasin D (CyD) on the level of SNARE complexes in rat parotid glands. When SNARE complexes were immunoprecipitated by anti-SNAP23, the coprecipitation of VAMP2 was significantly increased in response to ISO and/or CyD, although the coprecipitation of VAMP8 or syntaxin 4 was scarcely augmented. These results suggest that the SNAP23-VAMP2 interaction plays a key role in cAMP-mediated exocytosis from parotid glands. Structured summary of protein interactions: Snap23 physically interacts with Vamp8, Vamp3 and Syn-4 by anti bait coimmunoprecipitation (View Interaction: 1, 2, 3) Vamp2 physically interacts with Syn-4, Syn-3 and Snap23 by anti bait coimmunoprecipitation (View interaction) Syn-3 physically interacts with Snap23 by anti bait coimmunoprecipitation (View interaction) Vamp2 physically interacts with Snap23 and Syn-4 by anti bait coimmunoprecipitation (View interaction) Snap23 physically interacts with Vamp8, Syn-4 and Vamp2 by anti bait coimmunoprecipitation (View interaction: 1, 2).

Original language	English
Pages (from-to)	583-589
Number of pages	7
Journal	FEBS Letters
Volume	587
Issue number	6
DOIs	https://doi.org/10.1016/j.febslet.2013.01.039
Publication status	Published - 2013 Mar 18

Keywords

Cytochalasin D
Exocytosis
Parotid gland
SNAP23
Soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE)
VAMP2

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10.1016/j.febslet.2013.01.039

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@article{74b05b4b5251487bbd6d03aff640eb66,

title = "Isoproterenol stimulates transient SNAP23-VAMP2 interaction in rat parotid glands",

abstract = "The exocytosis of salivary proteins is mainly regulated by cAMP, although soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs), which mediate cAMP-dependent exocytic membrane fusion, have remained unidentified. Here we examined the effect of isoproterenol (ISO) and cytochalasin D (CyD) on the level of SNARE complexes in rat parotid glands. When SNARE complexes were immunoprecipitated by anti-SNAP23, the coprecipitation of VAMP2 was significantly increased in response to ISO and/or CyD, although the coprecipitation of VAMP8 or syntaxin 4 was scarcely augmented. These results suggest that the SNAP23-VAMP2 interaction plays a key role in cAMP-mediated exocytosis from parotid glands. Structured summary of protein interactions: Snap23 physically interacts with Vamp8, Vamp3 and Syn-4 by anti bait coimmunoprecipitation (View Interaction: 1, 2, 3) Vamp2 physically interacts with Syn-4, Syn-3 and Snap23 by anti bait coimmunoprecipitation (View interaction) Syn-3 physically interacts with Snap23 by anti bait coimmunoprecipitation (View interaction) Vamp2 physically interacts with Snap23 and Syn-4 by anti bait coimmunoprecipitation (View interaction) Snap23 physically interacts with Vamp8, Syn-4 and Vamp2 by anti bait coimmunoprecipitation (View interaction: 1, 2).",

keywords = "Cytochalasin D, Exocytosis, Parotid gland, SNAP23, Soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE), VAMP2",

author = "Taishin Takuma and Akiko Shitara and Toshiya Arakawa and Miki Okayama and Itaru Mizoguchi and Yoshifumi Tajima",

note = "Funding Information: This study was supported in part by HAITEKU from the Ministry of Education, Culture, Sports Science and Technology of Japan .",

year = "2013",

month = mar,

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doi = "10.1016/j.febslet.2013.01.039",

language = "English",

volume = "587",

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T1 - Isoproterenol stimulates transient SNAP23-VAMP2 interaction in rat parotid glands

AU - Takuma, Taishin

AU - Shitara, Akiko

AU - Arakawa, Toshiya

AU - Okayama, Miki

AU - Mizoguchi, Itaru

AU - Tajima, Yoshifumi

N1 - Funding Information: This study was supported in part by HAITEKU from the Ministry of Education, Culture, Sports Science and Technology of Japan .

PY - 2013/3/18

Y1 - 2013/3/18

N2 - The exocytosis of salivary proteins is mainly regulated by cAMP, although soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs), which mediate cAMP-dependent exocytic membrane fusion, have remained unidentified. Here we examined the effect of isoproterenol (ISO) and cytochalasin D (CyD) on the level of SNARE complexes in rat parotid glands. When SNARE complexes were immunoprecipitated by anti-SNAP23, the coprecipitation of VAMP2 was significantly increased in response to ISO and/or CyD, although the coprecipitation of VAMP8 or syntaxin 4 was scarcely augmented. These results suggest that the SNAP23-VAMP2 interaction plays a key role in cAMP-mediated exocytosis from parotid glands. Structured summary of protein interactions: Snap23 physically interacts with Vamp8, Vamp3 and Syn-4 by anti bait coimmunoprecipitation (View Interaction: 1, 2, 3) Vamp2 physically interacts with Syn-4, Syn-3 and Snap23 by anti bait coimmunoprecipitation (View interaction) Syn-3 physically interacts with Snap23 by anti bait coimmunoprecipitation (View interaction) Vamp2 physically interacts with Snap23 and Syn-4 by anti bait coimmunoprecipitation (View interaction) Snap23 physically interacts with Vamp8, Syn-4 and Vamp2 by anti bait coimmunoprecipitation (View interaction: 1, 2).

AB - The exocytosis of salivary proteins is mainly regulated by cAMP, although soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs), which mediate cAMP-dependent exocytic membrane fusion, have remained unidentified. Here we examined the effect of isoproterenol (ISO) and cytochalasin D (CyD) on the level of SNARE complexes in rat parotid glands. When SNARE complexes were immunoprecipitated by anti-SNAP23, the coprecipitation of VAMP2 was significantly increased in response to ISO and/or CyD, although the coprecipitation of VAMP8 or syntaxin 4 was scarcely augmented. These results suggest that the SNAP23-VAMP2 interaction plays a key role in cAMP-mediated exocytosis from parotid glands. Structured summary of protein interactions: Snap23 physically interacts with Vamp8, Vamp3 and Syn-4 by anti bait coimmunoprecipitation (View Interaction: 1, 2, 3) Vamp2 physically interacts with Syn-4, Syn-3 and Snap23 by anti bait coimmunoprecipitation (View interaction) Syn-3 physically interacts with Snap23 by anti bait coimmunoprecipitation (View interaction) Vamp2 physically interacts with Snap23 and Syn-4 by anti bait coimmunoprecipitation (View interaction) Snap23 physically interacts with Vamp8, Syn-4 and Vamp2 by anti bait coimmunoprecipitation (View interaction: 1, 2).

KW - Cytochalasin D

KW - Exocytosis

KW - Parotid gland

KW - SNAP23

KW - Soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE)

KW - VAMP2

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DO - 10.1016/j.febslet.2013.01.039

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C2 - 23380067

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SN - 0014-5793

VL - 587

SP - 583

EP - 589

JO - FEBS Letters

JF - FEBS Letters

IS - 6

ER -

Isoproterenol stimulates transient SNAP23-VAMP2 interaction in rat parotid glands

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Keywords

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