TY - JOUR
T1 - Jacaric acid, a linolenic acid isomer with a conjugated triene system, has a strong antitumor effect in vitro and in vivo
AU - Shinohara, Nahoko
AU - Tsuduki, Tsuyoshi
AU - Ito, Junya
AU - Honma, Taro
AU - Kijima, Ryo
AU - Sugawara, Soko
AU - Arai, Tatsuya
AU - Yamasaki, Masao
AU - Ikezaki, Aya
AU - Yokoyama, Marino
AU - Nishiyama, Kazuo
AU - Nakagawa, Kiyotaka
AU - Miyazawa, Teruo
AU - Ikeda, Ikuo
N1 - Funding Information:
This study was supported by KAKENHI of JSPS, Japan ( 20157639 ). The authors declare that there are no conflicts of interest.
PY - 2012/7
Y1 - 2012/7
N2 - In this study, we compared the cytotoxic effects of natural conjugated linolenic acids (CLnAs) on human adenocarcinoma cells (DLD-1) in vitro, with the goal of finding CLnA isomers with strong cytotoxic effects. The antitumor effect of the CLnA with the strongest cytotoxic effect was then examined in mice. The results showed that all CLnA isomers have strong cytotoxic effects on DLD-1 cells, with jacaric acid (JA) having the strongest effect. Examination of the mechanism of cell death showed that CLnAs induce apoptosis in DLD-1 cells via lipid peroxidation. The intracellular levels of incorporated CLnAs were measured to examine the reason for differences in cytotoxic effects. These results showed that JA was taken into cells efficiently. Collectively, these results suggest that the cytotoxic effect of CLnAs is dependent on intracellular incorporation and induction of apoptosis via lipid peroxidation. JA also had a strong preventive antitumor effect in vivo in nude mice into which DLD-1 cells were transplanted. These results suggest that JA can be used as a dietary constituent for prevention of cancer.
AB - In this study, we compared the cytotoxic effects of natural conjugated linolenic acids (CLnAs) on human adenocarcinoma cells (DLD-1) in vitro, with the goal of finding CLnA isomers with strong cytotoxic effects. The antitumor effect of the CLnA with the strongest cytotoxic effect was then examined in mice. The results showed that all CLnA isomers have strong cytotoxic effects on DLD-1 cells, with jacaric acid (JA) having the strongest effect. Examination of the mechanism of cell death showed that CLnAs induce apoptosis in DLD-1 cells via lipid peroxidation. The intracellular levels of incorporated CLnAs were measured to examine the reason for differences in cytotoxic effects. These results showed that JA was taken into cells efficiently. Collectively, these results suggest that the cytotoxic effect of CLnAs is dependent on intracellular incorporation and induction of apoptosis via lipid peroxidation. JA also had a strong preventive antitumor effect in vivo in nude mice into which DLD-1 cells were transplanted. These results suggest that JA can be used as a dietary constituent for prevention of cancer.
KW - Conjugated fatty acid
KW - Conjugated linolenic acid
KW - DLD-1
KW - Jacaranda seed oil
KW - Jacaric acid
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U2 - 10.1016/j.bbalip.2012.04.001
DO - 10.1016/j.bbalip.2012.04.001
M3 - Article
C2 - 22521763
AN - SCOPUS:84860660557
SN - 1388-1981
VL - 1821
SP - 980
EP - 988
JO - BBA - Specialised Section On Lipids and Related Subjects
JF - BBA - Specialised Section On Lipids and Related Subjects
IS - 7
ER -
