TY - JOUR
T1 - Janus-Type Immunofluorescent Probes and a Quantitative Immunoassay System
AU - Yabu, Hiroshi
AU - Suzuki, Misako
AU - Matsukawa, Kazushige
AU - Maeda, Ikuma
AU - Ihara, Sigeo
AU - Yaegashi, Koshiro
AU - Totsu, Kentaro
AU - Hayashi, Hironori
AU - Kodama, Eiichi
N1 - Publisher Copyright:
© 2024 American Chemical Society.
PY - 2024
Y1 - 2024
N2 - To realize highly sensitive immunoassays, high optical density probes conjugated with antibodies for target antigens have been demanded in order to increase the visibility of antigen-antibody complex formation. We herein demonstrate the development of an immunoassay system using magnetic and fluorescent Janus particles as probes in conjunction with an antibody-immobilized microfluidic device. The concentration of the detection limit at which there was a significant difference between SARS-CoV-2 and human coronavirus 229E antigens was 3.1 ng/mL, and the standard deviation of the signal was less than 5%. The immunofluorescent probe and immunoassay system developed in this study are expected to be applicable not only to SARS-CoV-2 but also to the quantitative measurement of various other disease marker proteins and biomolecules.
AB - To realize highly sensitive immunoassays, high optical density probes conjugated with antibodies for target antigens have been demanded in order to increase the visibility of antigen-antibody complex formation. We herein demonstrate the development of an immunoassay system using magnetic and fluorescent Janus particles as probes in conjunction with an antibody-immobilized microfluidic device. The concentration of the detection limit at which there was a significant difference between SARS-CoV-2 and human coronavirus 229E antigens was 3.1 ng/mL, and the standard deviation of the signal was less than 5%. The immunofluorescent probe and immunoassay system developed in this study are expected to be applicable not only to SARS-CoV-2 but also to the quantitative measurement of various other disease marker proteins and biomolecules.
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U2 - 10.1021/acs.langmuir.4c01911
DO - 10.1021/acs.langmuir.4c01911
M3 - Article
AN - SCOPUS:85201484881
SN - 0743-7463
JO - Langmuir
JF - Langmuir
ER -
