Kinetics of conformational sampling in ubiquitin

David Ban; Michael Funk; Rudolf Gulich; Dalia Egger; T. Michael Sabo; Korvin F.A. Walter; R. Bryn Fenwick; Karin Giller; Fabio Pichierri; Bert L. Degroot; Oliver F. Lange; Helmut Grubmüller; Xavier Salvatella; Martin Wolf; Alois Loidl; Reiner Kree; Stefan Becker; Nils Alexander Lakomek; Donghan Lee; Peter Lunkenheimer; Christian Griesinger

doi:10.1002/anie.201105086

Kinetics of conformational sampling in ubiquitin

David Ban, Michael Funk, Rudolf Gulich, Dalia Egger, T. Michael Sabo, Korvin F.A. Walter, R. Bryn Fenwick, Karin Giller, Fabio Pichierri, Bert L. Degroot, Oliver F. Lange, Helmut Grubmüller, Xavier Salvatella, Martin Wolf, Alois Loidl, Reiner Kree, Stefan Becker, Nils Alexander Lakomek, Donghan Lee, Peter LunkenheimerChristian Griesinger

ENG - Chemical Engineering

Research output: Contribution to journal › Article › peer-review

59 Citations (Scopus)

Abstract

A good combination: The lifetime of interconversion among the ground states of a protein ensemble representation could only be assigned to a time window that is four orders of magnitude large and ranges from 4ns to 50 s. By combining temperature-dependent NMR relaxation dispersion (RD) experiments and dielectric relaxation (DR) spectroscopy in solution, the lifetime was now identified to a value of (10±9) s at 309K.

Original language	English
Pages (from-to)	11437-11440
Number of pages	4
Journal	Angewandte Chemie - International Edition
Volume	50
Issue number	48
DOIs	https://doi.org/10.1002/anie.201105086
Publication status	Published - 2011 Nov 25

Keywords

NMR spectroscopy
dielectric spectroscopy
kinetics
proteins
relaxation dispersion

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10.1002/anie.201105086

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Ban, D., Funk, M., Gulich, R., Egger, D., Sabo, T. M., Walter, K. F. A., Fenwick, R. B., Giller, K., Pichierri, F., Degroot, B. L., Lange, O. F., Grubmüller, H., Salvatella, X., Wolf, M., Loidl, A., Kree, R., Becker, S., Lakomek, N. A., Lee, D., ... Griesinger, C. (2011). Kinetics of conformational sampling in ubiquitin. Angewandte Chemie - International Edition, 50(48), 11437-11440. https://doi.org/10.1002/anie.201105086

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abstract = "A good combination: The lifetime of interconversion among the ground states of a protein ensemble representation could only be assigned to a time window that is four orders of magnitude large and ranges from 4ns to 50 s. By combining temperature-dependent NMR relaxation dispersion (RD) experiments and dielectric relaxation (DR) spectroscopy in solution, the lifetime was now identified to a value of (10±9) s at 309K.",

keywords = "NMR spectroscopy, dielectric spectroscopy, kinetics, proteins, relaxation dispersion",

author = "David Ban and Michael Funk and Rudolf Gulich and Dalia Egger and Sabo, {T. Michael} and Walter, {Korvin F.A.} and Fenwick, {R. Bryn} and Karin Giller and Fabio Pichierri and Degroot, {Bert L.} and Lange, {Oliver F.} and Helmut Grubm{\"u}ller and Xavier Salvatella and Martin Wolf and Alois Loidl and Reiner Kree and Stefan Becker and Lakomek, {Nils Alexander} and Donghan Lee and Peter Lunkenheimer and Christian Griesinger",

year = "2011",

month = nov,

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doi = "10.1002/anie.201105086",

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Ban, D, Funk, M, Gulich, R, Egger, D, Sabo, TM, Walter, KFA, Fenwick, RB, Giller, K, Pichierri, F, Degroot, BL, Lange, OF, Grubmüller, H, Salvatella, X, Wolf, M, Loidl, A, Kree, R, Becker, S, Lakomek, NA, Lee, D, Lunkenheimer, P & Griesinger, C 2011, 'Kinetics of conformational sampling in ubiquitin', Angewandte Chemie - International Edition, vol. 50, no. 48, pp. 11437-11440. https://doi.org/10.1002/anie.201105086

TY - JOUR

T1 - Kinetics of conformational sampling in ubiquitin

AU - Ban, David

AU - Funk, Michael

AU - Gulich, Rudolf

AU - Egger, Dalia

AU - Sabo, T. Michael

AU - Walter, Korvin F.A.

AU - Fenwick, R. Bryn

AU - Giller, Karin

AU - Pichierri, Fabio

AU - Degroot, Bert L.

AU - Lange, Oliver F.

AU - Grubmüller, Helmut

AU - Salvatella, Xavier

AU - Wolf, Martin

AU - Loidl, Alois

AU - Kree, Reiner

AU - Becker, Stefan

AU - Lakomek, Nils Alexander

AU - Lee, Donghan

AU - Lunkenheimer, Peter

AU - Griesinger, Christian

PY - 2011/11/25

Y1 - 2011/11/25

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KW - NMR spectroscopy

KW - dielectric spectroscopy

KW - kinetics

KW - proteins

KW - relaxation dispersion

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JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

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Kinetics of conformational sampling in ubiquitin

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Keywords

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