Kinetochore capture and bi-orientation on the mitotic spindle

Tomoyuki U. Tanaka, Michael J.R. Stark, Kozo Tanaka

Research output: Contribution to journalReview articlepeer-review

92 Citations (Scopus)


Kinetochores are large protein complexes that are formed on chromosome regions known as centromeres. For high-fidelity chromosome segregation, kinetochores must be correctly captured on the mitotic spindle before anaphase onset. During prometaphase, kinetochores are initially captured by a single microtubule that extends from a spindle pole and are then transported poleward along the microtubule. Subsequently, microtubules that extend from the other spindle pole also interact with kinetochores and, eventually, each sister kinetochore attaches to microtubules that extend from opposite poles - this is known as bi-orientation. Here we discuss the molecular mechanisms of these processes, by focusing on budding yeast and drawing comparisons with other organisms.

Original languageEnglish
Pages (from-to)929-942
Number of pages14
JournalNature Reviews Molecular Cell Biology
Issue number12
Publication statusPublished - 2005 Dec


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