TY - JOUR
T1 - Kv1.2-immunoreactive primary sensory neurons in the rat trigeminal ganglion
AU - Ichikawa, Hiroyuki
AU - Sugimoto, Tomosada
N1 - Funding Information:
This work was supported by a grant from the Japanese Ministry of Education, Culture, Sports, Science and Technology to H.I. (No. 14571733).
PY - 2003/6/6
Y1 - 2003/6/6
N2 - Immunohistochemistry for Kv1.2, a subunit of voltage-gated K+ channels, was performed on the trigeminal ganglion (TG). Immunoreactivity (ir) was detected in half (48%) the TG neurons. These neurons were mostly medium-sized to large (range 137.6-2664.8 μm2, mean±S.D. 892.6±413.3 μm2). A double immunofluorescence method also revealed co-expression of Kv1.2 and parvalbumin. Half (54%) the Kv1.2-immunoreactive (ir) neurons exhibited parvalbumin-ir, and parvalbumin-ir neurons mostly showed Kv1.2-ir (95%). Kv1.2-ir neurons which co-expressed CGRP-ir were rare in this ganglion. Some 40% of TG neurons retrogradely labeled from the facial skin exhibited Kv1.2-ir, whereas ir was detected in 16% of those labeled from the tooth pulp. The present study indicates that Kv1.2-ir TG neurons include low-threshold mechanoreceptors and nociceptors which innervate the facial skin and tooth pulp, respectively.
AB - Immunohistochemistry for Kv1.2, a subunit of voltage-gated K+ channels, was performed on the trigeminal ganglion (TG). Immunoreactivity (ir) was detected in half (48%) the TG neurons. These neurons were mostly medium-sized to large (range 137.6-2664.8 μm2, mean±S.D. 892.6±413.3 μm2). A double immunofluorescence method also revealed co-expression of Kv1.2 and parvalbumin. Half (54%) the Kv1.2-immunoreactive (ir) neurons exhibited parvalbumin-ir, and parvalbumin-ir neurons mostly showed Kv1.2-ir (95%). Kv1.2-ir neurons which co-expressed CGRP-ir were rare in this ganglion. Some 40% of TG neurons retrogradely labeled from the facial skin exhibited Kv1.2-ir, whereas ir was detected in 16% of those labeled from the tooth pulp. The present study indicates that Kv1.2-ir TG neurons include low-threshold mechanoreceptors and nociceptors which innervate the facial skin and tooth pulp, respectively.
KW - Calcitonin gene-related peptide
KW - Facial skin
KW - Immunohistochemistry
KW - Kv1.2
KW - Parvalbumin
KW - Tooth pulp
KW - Trigeminal ganglion
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U2 - 10.1016/S0006-8993(03)02583-6
DO - 10.1016/S0006-8993(03)02583-6
M3 - Article
C2 - 12742640
AN - SCOPUS:0037651089
SN - 0006-8993
VL - 974
SP - 222
EP - 227
JO - Molecular Brain Research
JF - Molecular Brain Research
IS - 1-2
ER -