Label-free detection of DNA molecules moving in micro-fluidic channels by infrared absorption spectroscopy

Tomoyuki Miyoshi, Ken ichi Ishibashi, Koichiro Miyamoto, Ayumi Hirano-Iwata, Yasuo Kimura, Michio Niwano

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Infrared absorption spectroscopy (IRAS) in a multiple internal reflection (MIR) geometry is integrated with silicon-based micro-fluidic channels to allow detection and separation of DNA molecules in the micro-channels. The micro-channels were fabricated on a Si MIR-prism using the conventional silicon lithographic techniques. In the present method we can probe solution-phase biomolecular species through IR spectral profiles of those biomolecules, allowing us to detect and separate biomolecules without fluorescence labeling. The applicability of this approach is demonstrated with a representative test case: transport of single-stranded DNA molecules (oligonucleotides) in the micro-fluidic channels.

Original languageEnglish
Pages (from-to)917-922
Number of pages6
JournalSensors and Actuators B: Chemical
Volume238
DOIs
Publication statusPublished - 2017 Jan 1

Keywords

  • DNA
  • Infrared spectroscopy
  • Micro-fluidic channel

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