Lanthanide-based protease activity sensors for time-resolved fluorescence measurements

Shin Mizukami, Kazuhiro Tonai, Masahiro Kaneko, Kazuya Kikuchi

Research output: Contribution to journalArticlepeer-review

108 Citations (Scopus)


Lanthanide-based luminescent sensors are noteworthy because their long-lifetime luminescence enables time-resolved fluorescence measurements. After exploring suitable antenna groups, we designed and synthesized lanthanide-based luminogenic sensors detecting protease activities. This sensor yielded strong luminescence on addition of proteases such as calpain I and leucine aminopeptidase (LAP). Since the luminescence lifetimes of the probes were very long, the sensors could be applied to time-resolved measurements that exclude background fluorescence signals derived from proteins or other impurities. This sensing principle could be applicable to general time-resolved fluorescence assays for other proteases.

Original languageEnglish
Pages (from-to)14376-14377
Number of pages2
JournalJournal of the American Chemical Society
Issue number44
Publication statusPublished - 2008 Nov 5


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