@article{723f16971d3e4a478931a153cf651ddc,
title = "Levels of periplasmic nitrate reductase during denitrification are lower in Bradyrhizobium japonicum than in Bradyrhizobium diazoefficiens",
abstract = "Soybean plants host endosymbiotic dinitrogen (N2)-fixing bacteria from the genus Bradyrhizobium. Under oxygen-limiting conditions, Bradyrhizobium diazoefficiens and Bradyrhizobium japonicum perform denitrification by sequentially reducing nitrate (NO3–) to nitrous oxide (N2O) or N2. The anaerobic reduction of NO3– to N2O was previously shown to be lower in B. japonicum than in B. diazoefficiens due to impaired periplasmic nitrate reductase (Nap) activity in B. japonicum. We herein demonstrated that impaired Nap activity in B. japonicum was due to low Nap protein levels, which may be related to a decline in the production of FixP and FixO proteins by the cbb3-type oxidase.",
keywords = "Bradyrhizobium, Cbb oxidase, Competitive growth, Denitrification, Periplasmic nitrate reductase",
author = "Siqueira, {Arthur Fernandes} and Masayuki Sugawara and Haruka Arashida and Kiwamu Minamisawa and Cristina S{\'a}nchez",
note = "Funding Information: We thank Hans-Martin Fischer (Institute of Microbiology, ETH Z{\"u}rich, Switzerland) and Hiroshi Kouchi (International Christian University, Tokyo, Japan) for kindly providing the plasmids, pRJPaph-gfp and pBjGroEL4::DsRed2, respectively. This work was supported by Grants-in-Aid for Scientific Research (A) 26252065 and (B) 18H02112 from the Ministry of Education, Cul-ture, Sports, Science and Technology of Japan, and by grants from the Project of the NARO Bio-oriented Technology Research Advancement Institution (Research Program on Development of Innovative Technology). A.F.S. was supported by fellowships from the National Council for Scientific and Technological Development-Brazil and the Japan Society for the Promotion of Science. Funding Information: We thank Hans-Martin Fischer (Institute of Microbiology, ETH Z{\"u}rich, Switzerlan?) an? Hiroshi Kouchi (International Christian University, Tokyo, Japan) for kin?ly provi?ing the plasmi?s, pRJPaph-gfp an? pBjGroEL4::DsRe?2, respectively. This work was supporte? by Grants-in-Ai? for Scientific Research (A) 26252065 an? (B) 18H02112 from the Ministry of E?ucation, Cul-E ture, Sports, Science an? Technology of Japan, an? by grants from the Project of the NARO Bio-oriente? Technology Research A?vancement Institution (Research Program on Development of Innovative Technology). A.F.S. was supporte? by fellowships from the National Council for Scientific an? Technological Development-Brazil an? the Japan Society for the Promotion of Science. Publisher Copyright: {\textcopyright} 2020, Japanese Society of Microbial Ecology. All rights reserved.",
year = "2020",
doi = "10.1264/jsme2.ME19129",
language = "English",
volume = "35",
pages = "1--5",
journal = "Microbes and Environments",
issn = "1342-6311",
publisher = "Japanese Society Of Microbial Ecology",
number = "3",
}