TY - JOUR
T1 - LGR4 is required for the cell survival of the peripheral mesenchyme at the embryonic stages of nephrogenesis
AU - Mohri, Yasuaki
AU - Oyama, Kazunori
AU - Sone, Mizuki
AU - Akamatsu, Atsushi
AU - Nishimori, Katsuhiko
N1 - Funding Information:
Y.M. was supported by the Research Fellowships of the Japan Society for the Promotion of Science for Young Scientists.
PY - 2012
Y1 - 2012
N2 - In mice, homozygous Lgr4 inactivation results in hypoplastic kidneys. To understand better the role of LGR4 in kidney development, we performed an analysis of kidneys in Lgr4-/- embryos. We stained Lgr4-/- kidneys with anti-WT1 and anti-Cleaved Caspase3 antibodies at E16.5, and observed that the structures of the cap mesenchyme were disrupted and that apoptosis increased. In addition, the expression of PAX2, an anti-apoptotic factor in kidney development, was also significantly decreased at E16.5. We found that the LGR4 defect caused an increase in apoptosis in the peripheral mesenchyme during kidney development.
AB - In mice, homozygous Lgr4 inactivation results in hypoplastic kidneys. To understand better the role of LGR4 in kidney development, we performed an analysis of kidneys in Lgr4-/- embryos. We stained Lgr4-/- kidneys with anti-WT1 and anti-Cleaved Caspase3 antibodies at E16.5, and observed that the structures of the cap mesenchyme were disrupted and that apoptosis increased. In addition, the expression of PAX2, an anti-apoptotic factor in kidney development, was also significantly decreased at E16.5. We found that the LGR4 defect caused an increase in apoptosis in the peripheral mesenchyme during kidney development.
KW - Apoptosis
KW - Kidney morphogenesis
KW - Knockout mice
KW - LGR4
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U2 - 10.1271/bbb.110834
DO - 10.1271/bbb.110834
M3 - Article
C2 - 22738954
AN - SCOPUS:84861453168
SN - 0916-8451
VL - 76
SP - 888
EP - 891
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
IS - 5
ER -