TY - JOUR
T1 - Ligand-directed tosyl chemistry for protein labeling in vivo
AU - Tsukiji, Shinya
AU - Miyagawa, Masayoshi
AU - Takaoka, Yousuke
AU - Tamura, Tomonori
AU - Hamachi, Itaru
N1 - Funding Information:
We thank M. Shirakawa and H. Tochio (Kyoto University) for helpful discussions and assistance with 19F NMR measurements. We are grateful to Y. Mori and all members of his group (Kyoto University), in particular S. Kiyonaka, Y. Uriu and S. Yamamoto, for generous assistance on animal experiments. We thank E. Ashihara (Kyoto University Hospital) for his help in taking blood samples. We thank M. Yamada (Shimadzu Corporation) for measuring tandem mass spectra (MALDI-QIT-TOF MS/MS). We also thank all members of the Hamachi laboratory, particularly H. Nonaka, for discussions and experimental assistance. Y.T. acknowledges the Japan Society for the Promotion of Science Research Fellowships for Young Scientists.
PY - 2009/5
Y1 - 2009/5
N2 - Here we describe a method for the site-selective attachment of synthetic molecules into specific 'endogenous' proteins in vivo using ligand-directed tosyl (LDT) chemistry. This approach was applied not only for chemically labeling proteins in living cells, tissues and mice but also for constructing a biosensor directly inside cells without genetic engineering. These data establish LDT chemistry as a new tool for the study and manipulation of biological systems.
AB - Here we describe a method for the site-selective attachment of synthetic molecules into specific 'endogenous' proteins in vivo using ligand-directed tosyl (LDT) chemistry. This approach was applied not only for chemically labeling proteins in living cells, tissues and mice but also for constructing a biosensor directly inside cells without genetic engineering. These data establish LDT chemistry as a new tool for the study and manipulation of biological systems.
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U2 - 10.1038/nchembio.157
DO - 10.1038/nchembio.157
M3 - Article
C2 - 19330012
AN - SCOPUS:65349110554
SN - 1552-4450
VL - 5
SP - 341
EP - 343
JO - Nature Chemical Biology
JF - Nature Chemical Biology
IS - 5
ER -