Ligand-directed tosyl chemistry for protein labeling in vivo

Shinya Tsukiji, Masayoshi Miyagawa, Yousuke Takaoka, Tomonori Tamura, Itaru Hamachi

Research output: Contribution to journalArticlepeer-review

267 Citations (Scopus)


Here we describe a method for the site-selective attachment of synthetic molecules into specific 'endogenous' proteins in vivo using ligand-directed tosyl (LDT) chemistry. This approach was applied not only for chemically labeling proteins in living cells, tissues and mice but also for constructing a biosensor directly inside cells without genetic engineering. These data establish LDT chemistry as a new tool for the study and manipulation of biological systems.

Original languageEnglish
Pages (from-to)341-343
Number of pages3
JournalNature Chemical Biology
Issue number5
Publication statusPublished - 2009 May
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology


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