Ligand diversity contributes to the full activation of the jasmonate pathway in Marchantia polymorpha

Sophie Kneeshaw, Gonzalo Soriano, Isabel Monte, Mats Hamberg, Angel M. Zamarreño, Jose M. Garcıa-Mina, Jose Manuel Franco-Zorrilla, Nobuki Kato, Minoru Ueda, M. Fernanda Rey-Stolle, Coral Barbas, Santiago Michavila, Selena Gimenez-Ibanez, Guillermo H. Jimenez-Aleman, Roberto Solano

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)


In plants, jasmonate signaling regulates a wide range of processes from growth and development to defense responses and thermotolerance. Jasmonates, such as jasmonic acid (JA), (+)-7-iso-jasmonoyl-L-isoleucine (JA-Ile), 12-oxo-10,15(Z)-phytodienoic acid (OPDA), and dinor-12-oxo-10,15(Z)-phytodienoic acid (dn-OPDA), are derived from C18 (18 Carbon atoms) and C16 polyunsaturated fatty acids (PUFAs), which are found ubiquitously in the plant kingdom. Bryophytes are also rich in C20 and C22 long-chain polyunsaturated fatty acids (LCPUFAs), which are found only at low levels in some vascular plants but are abundant in organisms of other kingdoms, including animals. The existence of bioactive jasmonates derived from LCPUFAs is currently unknown. Here, we describe the identification of an OPDA-like molecule derived from a C20 fatty acid (FA) in the liverwort Marchantia polymorpha (Mp), which we term (5Z,8Z)-10-(4-oxo-5-((Z)-pent-2-en-1-yl)cyclopent-2-en-1-yl)deca-5,8-dienoic acid (C20-OPDA). This molecule accumulates upon wounding and, when applied exogenously, can activate known Coronatine Insensitive 1 (COI1) –dependent and –independent jasmonate responses. Furthermore, we identify a dn-OPDA–like molecule (Δ4-dn-OPDA) deriving from C20-OPDA and demonstrate it to be a ligand of the jasmonate coreceptor (MpCOI1–Mp Jasmonate-Zinc finger inflorescence meristem domain [MpJAZ]) in Marchantia. By analyzing mutants impaired in the production of LCPUFAs, we elucidate the major biosynthetic pathway of C20-OPDA and Δ4-dn-OPDA. Moreover, using a double mutant compromised in the production of both Δ4-dn-OPDA and dn-OPDA, we demonstrate the additive nature of these molecules in the activation of jasmonate responses. Taken together, our data identify a ligand of MpCOI1 and demonstrate LCPUFAs as a source of bioactive jasmonates that are essential to the immune response of M. polymorpha.

Original languageEnglish
Article numbere2202930119
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number36
Publication statusPublished - 2022 Sept 6


  • Marchantia
  • dn-OPDA
  • jasmonate
  • phytohormone
  • plant signaling

ASJC Scopus subject areas

  • General


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