Ligation errors in DNA computing

Yohei Aoi; Tatsuo Yoshinobu; Katsuyuki Tanizawa; Kozo Kinoshita; Hiroshi Iwasaki

doi:10.1016/S0303-2647(99)00045-3

Ligation errors in DNA computing

Yohei Aoi, Tatsuo Yoshinobu, Katsuyuki Tanizawa, Kozo Kinoshita, Hiroshi Iwasaki

MEN - Biomedical Engineering

Osaka University

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

DNA computing is a novel method of computing proposed by Adleman (1994), in which the data is encoded in the sequences of oligonucleotides. Massively parallel reactions between oligonucleotides are expected to make it possible to solve huge problems. In this study, reliability of the ligation process employed in the DNA computing is tested by estimating the error rate at which wrong oligonucleotides are ligated. Ligation of wrong oligonucleotides would result in a wrong answer in the DNA computing. The dependence of the error rate on the number of mismatches between oligonucleotides and on the combination of bases is investigated.

Original language	English
Pages (from-to)	181-187
Number of pages	7
Journal	BioSystems
Volume	52
Issue number	1-3
DOIs	https://doi.org/10.1016/S0303-2647(99)00045-3
Publication status	Published - 1999 Oct

Keywords

DNA computing
Electrophoresis
Ligation
Oligonucleotide

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10.1016/S0303-2647(99)00045-3

Cite this

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title = "Ligation errors in DNA computing",

abstract = "DNA computing is a novel method of computing proposed by Adleman (1994), in which the data is encoded in the sequences of oligonucleotides. Massively parallel reactions between oligonucleotides are expected to make it possible to solve huge problems. In this study, reliability of the ligation process employed in the DNA computing is tested by estimating the error rate at which wrong oligonucleotides are ligated. Ligation of wrong oligonucleotides would result in a wrong answer in the DNA computing. The dependence of the error rate on the number of mismatches between oligonucleotides and on the combination of bases is investigated.",

keywords = "DNA computing, Electrophoresis, Ligation, Oligonucleotide",

author = "Yohei Aoi and Tatsuo Yoshinobu and Katsuyuki Tanizawa and Kozo Kinoshita and Hiroshi Iwasaki",

note = "Funding Information: The authors are grateful to Dr R. Matsuzaki for his advice. This work was supported by the Center-of-Excellence Program (contract No. 09CE2005) and by a Grant-in-Aid for Exploratory Research from the Ministry of Education, Science, Sports and Culture (contract No. 08878043).",

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AU - Aoi, Yohei

AU - Yoshinobu, Tatsuo

AU - Tanizawa, Katsuyuki

AU - Kinoshita, Kozo

AU - Iwasaki, Hiroshi

N1 - Funding Information: The authors are grateful to Dr R. Matsuzaki for his advice. This work was supported by the Center-of-Excellence Program (contract No. 09CE2005) and by a Grant-in-Aid for Exploratory Research from the Ministry of Education, Science, Sports and Culture (contract No. 08878043).

PY - 1999/10

Y1 - 1999/10

N2 - DNA computing is a novel method of computing proposed by Adleman (1994), in which the data is encoded in the sequences of oligonucleotides. Massively parallel reactions between oligonucleotides are expected to make it possible to solve huge problems. In this study, reliability of the ligation process employed in the DNA computing is tested by estimating the error rate at which wrong oligonucleotides are ligated. Ligation of wrong oligonucleotides would result in a wrong answer in the DNA computing. The dependence of the error rate on the number of mismatches between oligonucleotides and on the combination of bases is investigated.

AB - DNA computing is a novel method of computing proposed by Adleman (1994), in which the data is encoded in the sequences of oligonucleotides. Massively parallel reactions between oligonucleotides are expected to make it possible to solve huge problems. In this study, reliability of the ligation process employed in the DNA computing is tested by estimating the error rate at which wrong oligonucleotides are ligated. Ligation of wrong oligonucleotides would result in a wrong answer in the DNA computing. The dependence of the error rate on the number of mismatches between oligonucleotides and on the combination of bases is investigated.

KW - DNA computing

KW - Electrophoresis

KW - Ligation

KW - Oligonucleotide

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Ligation errors in DNA computing

Abstract

Keywords

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