TY - JOUR
T1 - Light activation of expression associated with the tomato rbcS promoter in transformed tobacco cell line BY-2
AU - Uozumi, Nobuyuki
AU - Inoue, Yoshihisa
AU - Yamazaki, Ken ichi
AU - Kobayashi, Takeshi
N1 - Funding Information:
We are grateful to Dr. M. Sugita (Nagoya University) and Dr. W. Gruissem (University of California, Berkeley) for providing the plasmid, Rbcs-3B, and to Dr. K. Nakamura (Nagoya University) for providing the transformed BY-2 cell with cauliflower mosaic virus 35S promoter. We thank Mr. H. Onouchi and Dr. K. Matsuoka (Nagoya University) for technical advice and assistance in the transformation of A. tumefaciens and BY-2. This work was supported in part by a Grant-in-Aid for Scientific Research (No. 05855116) from the Ministry of Education, Science and Culture of Japan.
PY - 1994/7/29
Y1 - 1994/7/29
N2 - The promoter (2.3 kb) of ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit (rbcS3B) from tomato was inserted into the upstream multi-cloning site of β-glucuronidase (GUS) gene. The construction was introduced into the tobacco suspension cultured cell, BY-2. The transformed tobacco cell exhibited greater GUS activity (10-fold) under the light condition than the dark condition. The specific GUS activity increased with increasing culture time under the light condition. In fed-batch culture, high density culture was achieved, 47.6 g l-1 dry weight, which was 6.8-fold greater than the batch culture, whereas the GUS was produced 1.7-fold in specific activity compared with the batch culture. Growth of BY-2 and GUS gene expression during the culture were simulated by a kinetic model.
AB - The promoter (2.3 kb) of ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit (rbcS3B) from tomato was inserted into the upstream multi-cloning site of β-glucuronidase (GUS) gene. The construction was introduced into the tobacco suspension cultured cell, BY-2. The transformed tobacco cell exhibited greater GUS activity (10-fold) under the light condition than the dark condition. The specific GUS activity increased with increasing culture time under the light condition. In fed-batch culture, high density culture was achieved, 47.6 g l-1 dry weight, which was 6.8-fold greater than the batch culture, whereas the GUS was produced 1.7-fold in specific activity compared with the batch culture. Growth of BY-2 and GUS gene expression during the culture were simulated by a kinetic model.
KW - BY-2
KW - GUS activity
KW - Light inducible promoter
KW - Ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit
KW - Tobacco cell line
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U2 - 10.1016/0168-1656(94)90023-X
DO - 10.1016/0168-1656(94)90023-X
M3 - Article
C2 - 7765159
AN - SCOPUS:0027934495
SN - 0168-1656
VL - 36
SP - 55
EP - 62
JO - Journal of Biotechnology
JF - Journal of Biotechnology
IS - 1
ER -