TY - JOUR
T1 - Light-addressable potentiometric sensors for quantitative spatial imaging of chemical species
AU - Yoshinobu, Tatsuo
AU - Miyamoto, Ko Ichiro
AU - Werner, Carl Frederik
AU - Poghossian, Arshak
AU - Wagner, Torsten
AU - Schöning, Michael J.
N1 - Publisher Copyright:
© 2017 by Annual Reviews.
PY - 2017/6/12
Y1 - 2017/6/12
N2 - A light-addressable potentiometric sensor (LAPS) is a semiconductor-based chemical sensor, in which a measurement site on the sensing surface is defined by illumination. This light addressability can be applied to visualize the spatial distribution of pH or the concentration of a specific chemical species, with potential applications in the fields of chemistry, materials science, biology, and medicine. In this review, the features of this chemical imaging sensor technology are compared with those of other technologies. Instrumentation, principles of operation, and various measurement modes of chemical imaging sensor systems are described. The review discusses and summarizes state-of-the-art technologies, especially with regard to the spatial resolution and measurement speed; for example, a high spatial resolution in a submicron range and a readout speed in the range of several tens of thousands of pixels per second have been achieved with the LAPS. The possibility of combining this technology with microfluidic devices and other potential future developments are discussed.
AB - A light-addressable potentiometric sensor (LAPS) is a semiconductor-based chemical sensor, in which a measurement site on the sensing surface is defined by illumination. This light addressability can be applied to visualize the spatial distribution of pH or the concentration of a specific chemical species, with potential applications in the fields of chemistry, materials science, biology, and medicine. In this review, the features of this chemical imaging sensor technology are compared with those of other technologies. Instrumentation, principles of operation, and various measurement modes of chemical imaging sensor systems are described. The review discusses and summarizes state-of-the-art technologies, especially with regard to the spatial resolution and measurement speed; for example, a high spatial resolution in a submicron range and a readout speed in the range of several tens of thousands of pixels per second have been achieved with the LAPS. The possibility of combining this technology with microfluidic devices and other potential future developments are discussed.
KW - Bioimaging
KW - Biosensor
KW - Chemical imaging sensor
KW - LAPS
KW - PH sensor
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U2 - 10.1146/annurev-anchem-061516-045158
DO - 10.1146/annurev-anchem-061516-045158
M3 - Review article
C2 - 28375701
AN - SCOPUS:85020746714
SN - 1936-1327
VL - 10
SP - 225
EP - 246
JO - Annual Review of Analytical Chemistry
JF - Annual Review of Analytical Chemistry
ER -