The covalent modification of 2′-deoxycytidine (dCyd) by genotoxic bifunctional electrophiles, to produce unsubstituted and substituted etheno-dCyd adducts was discussed. The presence of DNA-adducts increased the probability of errors during DNA replication. The decomposition of 13- hydroperoxyoctadecadienoic acid (13-HPODE) was analyzed by reverse-phase chromatography with an acetonitrile/water system. The results of analysis show that the decomposition of 13-HPODE in the presence of dCyd by Liquid chromatography/atmospheric pressure chemical ionization/mass spectrometry revealed the formation of the unsubstituted 3,N 4-etheno-dCyd adduct and two substituted etheno-dCyd adducts.
|Number of pages
|Published - 2002
|Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: 2002 Jun 2 → 2002 Jun 6
|Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
|02/6/2 → 02/6/6