TY - JOUR
T1 - Lipidomic analysis of human tear fl uid reveals structure-specific lipid alterations in dry eye syndrome
AU - Lam, Sin Man
AU - Tong, Louis
AU - Reux, Bastien
AU - Duan, Xinrui
AU - Petznick, Andrea
AU - Yong, Siew Sian
AU - Khee, Cynthia Boo Shiao
AU - Lear, Martin J.
AU - Wenk, Markus R.
AU - Shui, Guanghou
PY - 2014/2
Y1 - 2014/2
N2 - As current diagnostic markers for dry eye syndrome (DES) are lacking in both sensitivity and specificity, a pressing concern exists to develop activity markers that closely align with the principal axes of disease progression. In this study, a comprehensive lipidomic platform designated for analysis of the human tear lipidome was employed to characterize changes in tear lipid compositions from a cohort of 93 subjects of different clinical subgroups classified based on the presence of dry eye symptoms and signs. Positive correlations were observed between the tear levels of cholesteryl sulfates and glycosphingolipids with physiological secretion of tears, which indicated the possible lacrimal (instead of meibomian) origin of these lipids. Notably, we found wax esters of low molecular masses and those containing saturated fatty acyl moieties were specifically reduced with disease and significantly correlated with various DES clinical parameters such as ocular surface disease index, tear breakup time, and Schirmer's I test (i.e., both symptoms and signs). These structure-specific changes in tear components with DES could potentially serve as unifying indicators of disease symptoms and signs. In addition, the structurally-specific aberrations in tear lipids reported here were found in patients with or without aqueous defi- ciency, suggesting a common pathology for both DES subtypes. -Lam, S. M., L. Tong, B. Reux, X. Duan, A. Petznick, S. S. Yong, C. B. S. Khee, M. J. Lear, M. R. Wenk, and G. Shui. Lipidomic analysis of human tear fl uid reveals structure-specific lipid alterations in dry eye syndrome.
AB - As current diagnostic markers for dry eye syndrome (DES) are lacking in both sensitivity and specificity, a pressing concern exists to develop activity markers that closely align with the principal axes of disease progression. In this study, a comprehensive lipidomic platform designated for analysis of the human tear lipidome was employed to characterize changes in tear lipid compositions from a cohort of 93 subjects of different clinical subgroups classified based on the presence of dry eye symptoms and signs. Positive correlations were observed between the tear levels of cholesteryl sulfates and glycosphingolipids with physiological secretion of tears, which indicated the possible lacrimal (instead of meibomian) origin of these lipids. Notably, we found wax esters of low molecular masses and those containing saturated fatty acyl moieties were specifically reduced with disease and significantly correlated with various DES clinical parameters such as ocular surface disease index, tear breakup time, and Schirmer's I test (i.e., both symptoms and signs). These structure-specific changes in tear components with DES could potentially serve as unifying indicators of disease symptoms and signs. In addition, the structurally-specific aberrations in tear lipids reported here were found in patients with or without aqueous defi- ciency, suggesting a common pathology for both DES subtypes. -Lam, S. M., L. Tong, B. Reux, X. Duan, A. Petznick, S. S. Yong, C. B. S. Khee, M. J. Lear, M. R. Wenk, and G. Shui. Lipidomic analysis of human tear fl uid reveals structure-specific lipid alterations in dry eye syndrome.
KW - Mass spectrometry
KW - Meibum
KW - Tear lipidome
KW - Wax esters
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U2 - 10.1194/jlr.P041780
DO - 10.1194/jlr.P041780
M3 - Article
C2 - 24287121
AN - SCOPUS:84893355643
SN - 0022-2275
VL - 55
SP - 299
EP - 306
JO - Journal of Lipid Research
JF - Journal of Lipid Research
IS - 2
ER -
