Liposomes destabilize tRNA during heat stress

Keishi Suga; Hiroshi Umakoshi; Hibiki Tomita; Tomoyuki Tanabe; Toshinori Shimanouchi; Ryoichi Kuboi

doi:10.1002/biot.200900289

Liposomes destabilize tRNA during heat stress

Keishi Suga, Hiroshi Umakoshi, Hibiki Tomita, Tomoyuki Tanabe, Toshinori Shimanouchi, Ryoichi Kuboi

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

Biomembranes play an important role in cellular response to heat stress. In this study, we focus on the interaction between liposomes and tRNA. Upon heat treatment we determined circular dichroism spectra of tRNA in presence of liposomes prepared from POPC (1-palmitoyl-2-oleoylsn-glycero-3-phosphocholine) and cholesterol (Ch). To compare thermal stability, midpoint temperature (T _m) of tRNA was calculated from normalized θ208. Addition of POPC/Ch liposomes decreased the T_m value of tRNA from 48°C to 38°C. We conclude that POPC/Ch liposomes interact with tRNA and destabilize its conformation under heat stress.

Original language	English
Pages (from-to)	526-529
Number of pages	4
Journal	Biotechnology Journal
Volume	5
Issue number	5
DOIs	https://doi.org/10.1002/biot.200900289
Publication status	Published - 2010 May
Externally published	Yes

Keywords

Biochemical engineering
Heat stress response
Liposome membrane stress biotechnology
tRNA conformation

ASJC Scopus subject areas

Applied Microbiology and Biotechnology
Molecular Medicine

Access to Document

10.1002/biot.200900289

Cite this

@article{c8d3487673ce45f0a0f49405aa691f9a,

title = "Liposomes destabilize tRNA during heat stress",

abstract = "Biomembranes play an important role in cellular response to heat stress. In this study, we focus on the interaction between liposomes and tRNA. Upon heat treatment we determined circular dichroism spectra of tRNA in presence of liposomes prepared from POPC (1-palmitoyl-2-oleoylsn-glycero-3-phosphocholine) and cholesterol (Ch). To compare thermal stability, midpoint temperature (T m) of tRNA was calculated from normalized θ208. Addition of POPC/Ch liposomes decreased the Tm value of tRNA from 48°C to 38°C. We conclude that POPC/Ch liposomes interact with tRNA and destabilize its conformation under heat stress.",

keywords = "Biochemical engineering, Heat stress response, Liposome membrane stress biotechnology, tRNA conformation",

author = "Keishi Suga and Hiroshi Umakoshi and Hibiki Tomita and Tomoyuki Tanabe and Toshinori Shimanouchi and Ryoichi Kuboi",

year = "2010",

month = may,

doi = "10.1002/biot.200900289",

language = "English",

volume = "5",

pages = "526--529",

journal = "Biotechnology Journal",

issn = "1860-6768",

publisher = "Wiley-VCH Verlag",

number = "5",

}

TY - JOUR

T1 - Liposomes destabilize tRNA during heat stress

AU - Suga, Keishi

AU - Umakoshi, Hiroshi

AU - Tomita, Hibiki

AU - Tanabe, Tomoyuki

AU - Shimanouchi, Toshinori

AU - Kuboi, Ryoichi

PY - 2010/5

Y1 - 2010/5

N2 - Biomembranes play an important role in cellular response to heat stress. In this study, we focus on the interaction between liposomes and tRNA. Upon heat treatment we determined circular dichroism spectra of tRNA in presence of liposomes prepared from POPC (1-palmitoyl-2-oleoylsn-glycero-3-phosphocholine) and cholesterol (Ch). To compare thermal stability, midpoint temperature (T m) of tRNA was calculated from normalized θ208. Addition of POPC/Ch liposomes decreased the Tm value of tRNA from 48°C to 38°C. We conclude that POPC/Ch liposomes interact with tRNA and destabilize its conformation under heat stress.

AB - Biomembranes play an important role in cellular response to heat stress. In this study, we focus on the interaction between liposomes and tRNA. Upon heat treatment we determined circular dichroism spectra of tRNA in presence of liposomes prepared from POPC (1-palmitoyl-2-oleoylsn-glycero-3-phosphocholine) and cholesterol (Ch). To compare thermal stability, midpoint temperature (T m) of tRNA was calculated from normalized θ208. Addition of POPC/Ch liposomes decreased the Tm value of tRNA from 48°C to 38°C. We conclude that POPC/Ch liposomes interact with tRNA and destabilize its conformation under heat stress.

KW - Biochemical engineering

KW - Heat stress response

KW - Liposome membrane stress biotechnology

KW - tRNA conformation

UR - http://www.scopus.com/inward/record.url?scp=77952134653&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77952134653&partnerID=8YFLogxK

U2 - 10.1002/biot.200900289

DO - 10.1002/biot.200900289

M3 - Article

C2 - 20401904

AN - SCOPUS:77952134653

SN - 1860-6768

VL - 5

SP - 526

EP - 529

JO - Biotechnology Journal

JF - Biotechnology Journal

IS - 5

ER -

Liposomes destabilize tRNA during heat stress

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this