TY - JOUR
T1 - Live-Cell Imaging and Optical Manipulation of Arabidopsis Early Embryogenesis
AU - Gooh, Keita
AU - Ueda, Minako
AU - Aruga, Kana
AU - Park, Jongho
AU - Arata, Hideyuki
AU - Higashiyama, Tetsuya
AU - Kurihara, Daisuke
N1 - Funding Information:
We thank R. Groß-Hardt, S. Takada, and W. Werr for the plant materials; S. Nasu, T. Nishii, and T. Shinagawa for assistance with cloning and generation of transgenic plants; and Y. Hamamura and N. Kaji for technical advice. Microscopy was conducted at the Institute of Transformative Bio-Molecules (WPI-ITbM) of Nagoya University and supported by the Japan Advanced Plant Science Network . This research was supported by grants from the Japan Science and Technology Agency (ERATO project to T.H. and M.U.) and the Ministry of Education, Culture, Sports, Science and Technology, Japan (no. 11025940 to D.K., no. 24113514 and 24770045 to M.U.).
Publisher Copyright:
© 2015 Elsevier Inc.
PY - 2015/7/27
Y1 - 2015/7/27
N2 - Intercellular communications are essential for cell proliferation and differentiation during plant embryogenesis. However, analysis of intercellular communications in living material in real time is difficult owing to the restricted accessibility of the embryo within the flower. We established a live-embryo imaging system to visualize cell division and cell fate specification in Arabidopsis thaliana from zygote division in real time. We generated a cell-division lineage tree for early embryogenesis in Arabidopsis. Lineage analysis showed that both the direction and time course of cell division between sister cells differed along the apical-basal or radial axes. Using the Arabidopsis kpl mutant, in which single-fertilization events are frequent, we showed that endosperm development is not required for pattern formation during early embryogenesis. Optical manipulation demonstrated that damage to the embryo initial cell induces cell fate conversion of the suspensor cell to compensate for the disrupted embryo initial cell even after cell fate is specified. Gooh et al. establish a live-embryo imaging system for Arabidopsis and generate a complete lineage tree from double fertilization in early embryogenesis. They provide a platform for real-time analysis of cell division dynamics and cell fate specification using optical manipulation and micro-engineering techniques such as laser irradiation of specific cells.
AB - Intercellular communications are essential for cell proliferation and differentiation during plant embryogenesis. However, analysis of intercellular communications in living material in real time is difficult owing to the restricted accessibility of the embryo within the flower. We established a live-embryo imaging system to visualize cell division and cell fate specification in Arabidopsis thaliana from zygote division in real time. We generated a cell-division lineage tree for early embryogenesis in Arabidopsis. Lineage analysis showed that both the direction and time course of cell division between sister cells differed along the apical-basal or radial axes. Using the Arabidopsis kpl mutant, in which single-fertilization events are frequent, we showed that endosperm development is not required for pattern formation during early embryogenesis. Optical manipulation demonstrated that damage to the embryo initial cell induces cell fate conversion of the suspensor cell to compensate for the disrupted embryo initial cell even after cell fate is specified. Gooh et al. establish a live-embryo imaging system for Arabidopsis and generate a complete lineage tree from double fertilization in early embryogenesis. They provide a platform for real-time analysis of cell division dynamics and cell fate specification using optical manipulation and micro-engineering techniques such as laser irradiation of specific cells.
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U2 - 10.1016/j.devcel.2015.06.008
DO - 10.1016/j.devcel.2015.06.008
M3 - Article
C2 - 26166301
AN - SCOPUS:84938091419
SN - 1534-5807
VL - 34
SP - 242
EP - 251
JO - Developmental Cell
JF - Developmental Cell
IS - 2
ER -