TY - JOUR
T1 - Live Cell imaging of endogenous mRNA Using RNA-Based fluorescence "turn-on" probe
AU - Ong, Wei Qiang
AU - Citron, Y. Rose
AU - Sekine, Sayaka
AU - Huang, Bo
N1 - Funding Information:
We thank M. C. Hammond, P. Yin, and A. A. Green for insightful discussions. We thank Y. Zhang and C. Gross for providing the E. coli RNA-seq data. This work was supported by the National Institutes of Health Director’s New Innovator Award (DP2OD008479) and the National Institutes of Health Extracellular RNA Communication Consortium (U19CA179512). Y.R.C. acknowledges support by the NSF Graduate Research Fellowship. S.S. thankfully acknowledges support by Japan Society for the Promotion of Science Postdoctoral Fellowship for Research Abroad.
Publisher Copyright:
© 2016 American Chemical Society.
PY - 2017/1/20
Y1 - 2017/1/20
N2 - Messenger RNA (mRNA) plays a critical role in cellular growth and development. However, there have been limited methods available to visualize endogenous mRNA in living cells with ease. We have designed RNA-based fluorescence "turn-on" probes that target mRNA by fusing an unstable form of Spinach with target-complementary sequences. These probes have been demonstrated to be selective, stable, and capable of targeting various mRNAs for live E. coli imaging.
AB - Messenger RNA (mRNA) plays a critical role in cellular growth and development. However, there have been limited methods available to visualize endogenous mRNA in living cells with ease. We have designed RNA-based fluorescence "turn-on" probes that target mRNA by fusing an unstable form of Spinach with target-complementary sequences. These probes have been demonstrated to be selective, stable, and capable of targeting various mRNAs for live E. coli imaging.
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U2 - 10.1021/acschembio.6b00586
DO - 10.1021/acschembio.6b00586
M3 - Article
C2 - 28103687
AN - SCOPUS:85026351477
SN - 1554-8929
VL - 12
SP - 200
EP - 205
JO - ACS Chemical Biology
JF - ACS Chemical Biology
IS - 1
ER -