Localization-dependent cell-killing effects of protoporphyrin (PPIX)-lipid micelles and liposomes in photodynamic therapy

Shoji Tachikawa; Shinichi Sato; Hisanao Hazama; Yasufumi Kaneda; Kunio Awazu; Hiroyuki Nakamura

doi:10.1016/j.bmc.2015.11.001

Localization-dependent cell-killing effects of protoporphyrin (PPIX)-lipid micelles and liposomes in photodynamic therapy

Shoji Tachikawa, Shinichi Sato, Hisanao Hazama, Yasufumi Kaneda, Kunio Awazu, Hiroyuki Nakamura

FRIS - Core Interdisciplinary Research Section

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

The protoporphyron (PPIX)-lipid (PL-C17) liposomes were successfully prepared from the corresponding micelles by post-inserted method. Both the PL-C17 micelles and liposomes were distributed in plasma membrane and cytoplasm after incubation of the cells with PL-C17 liposomes for 1 h. They translocated from plasma membrane into a certain organelle in the cells after incubation in the photosensitizer-free medium. Higher photo-cytotoxicity was observed in the PL-C17 micelles and liposomes localized in plasma membrane in comparison with those localized in the cytoplasm under light irradiation. The LDH assay revealed that cytopathic damages of the plasma membrane were observed in the PL-C17 micelles and liposomes highly localized in plasma membrane. The fluorescent intensity of the calcein-encapsulating DOPC liposomes post-inserted with PL-C17 increased after light irradiation, suggesting that the membrane disruption is possibly caused by oxidation of membrane lipids with ROS generated from photosensitizers and affects the photo-cytotoxicity in PDT.

Original language	English
Pages (from-to)	7578-7584
Number of pages	7
Journal	Bioorganic and Medicinal Chemistry
Volume	23
Issue number	24
DOIs	https://doi.org/10.1016/j.bmc.2015.11.001
Publication status	Published - 2015 Dec 15

Keywords

Drug delivery system (DDS)
Lipid
Liposome
Micelle
Photodynamic therapy (PDT)
Protoporphyrin IX (PPIX)
Reactive oxygen species (ROS)

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10.1016/j.bmc.2015.11.001

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title = "Localization-dependent cell-killing effects of protoporphyrin (PPIX)-lipid micelles and liposomes in photodynamic therapy",

abstract = "The protoporphyron (PPIX)-lipid (PL-C17) liposomes were successfully prepared from the corresponding micelles by post-inserted method. Both the PL-C17 micelles and liposomes were distributed in plasma membrane and cytoplasm after incubation of the cells with PL-C17 liposomes for 1 h. They translocated from plasma membrane into a certain organelle in the cells after incubation in the photosensitizer-free medium. Higher photo-cytotoxicity was observed in the PL-C17 micelles and liposomes localized in plasma membrane in comparison with those localized in the cytoplasm under light irradiation. The LDH assay revealed that cytopathic damages of the plasma membrane were observed in the PL-C17 micelles and liposomes highly localized in plasma membrane. The fluorescent intensity of the calcein-encapsulating DOPC liposomes post-inserted with PL-C17 increased after light irradiation, suggesting that the membrane disruption is possibly caused by oxidation of membrane lipids with ROS generated from photosensitizers and affects the photo-cytotoxicity in PDT.",

keywords = "Drug delivery system (DDS), Lipid, Liposome, Micelle, Photodynamic therapy (PDT), Protoporphyrin IX (PPIX), Reactive oxygen species (ROS)",

author = "Shoji Tachikawa and Shinichi Sato and Hisanao Hazama and Yasufumi Kaneda and Kunio Awazu and Hiroyuki Nakamura",

note = "Funding Information: This work was supported by the Ministry of Education, Science, Sports, Culture and Technology , Grant-in-Aid for Scientific Research (B) (No. 26293007 ) from Japan. We thank Professor Kunio Mochida (Gakushuin University) for his kind support to proceed light irradiation experiments using a xenon lamp. Publisher Copyright: {\textcopyright} 2015 Elsevier Ltd. All rights reserved.",

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doi = "10.1016/j.bmc.2015.11.001",

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T1 - Localization-dependent cell-killing effects of protoporphyrin (PPIX)-lipid micelles and liposomes in photodynamic therapy

AU - Tachikawa, Shoji

AU - Sato, Shinichi

AU - Hazama, Hisanao

AU - Kaneda, Yasufumi

AU - Awazu, Kunio

AU - Nakamura, Hiroyuki

N1 - Funding Information: This work was supported by the Ministry of Education, Science, Sports, Culture and Technology , Grant-in-Aid for Scientific Research (B) (No. 26293007 ) from Japan. We thank Professor Kunio Mochida (Gakushuin University) for his kind support to proceed light irradiation experiments using a xenon lamp. Publisher Copyright: © 2015 Elsevier Ltd. All rights reserved.

PY - 2015/12/15

Y1 - 2015/12/15

N2 - The protoporphyron (PPIX)-lipid (PL-C17) liposomes were successfully prepared from the corresponding micelles by post-inserted method. Both the PL-C17 micelles and liposomes were distributed in plasma membrane and cytoplasm after incubation of the cells with PL-C17 liposomes for 1 h. They translocated from plasma membrane into a certain organelle in the cells after incubation in the photosensitizer-free medium. Higher photo-cytotoxicity was observed in the PL-C17 micelles and liposomes localized in plasma membrane in comparison with those localized in the cytoplasm under light irradiation. The LDH assay revealed that cytopathic damages of the plasma membrane were observed in the PL-C17 micelles and liposomes highly localized in plasma membrane. The fluorescent intensity of the calcein-encapsulating DOPC liposomes post-inserted with PL-C17 increased after light irradiation, suggesting that the membrane disruption is possibly caused by oxidation of membrane lipids with ROS generated from photosensitizers and affects the photo-cytotoxicity in PDT.

AB - The protoporphyron (PPIX)-lipid (PL-C17) liposomes were successfully prepared from the corresponding micelles by post-inserted method. Both the PL-C17 micelles and liposomes were distributed in plasma membrane and cytoplasm after incubation of the cells with PL-C17 liposomes for 1 h. They translocated from plasma membrane into a certain organelle in the cells after incubation in the photosensitizer-free medium. Higher photo-cytotoxicity was observed in the PL-C17 micelles and liposomes localized in plasma membrane in comparison with those localized in the cytoplasm under light irradiation. The LDH assay revealed that cytopathic damages of the plasma membrane were observed in the PL-C17 micelles and liposomes highly localized in plasma membrane. The fluorescent intensity of the calcein-encapsulating DOPC liposomes post-inserted with PL-C17 increased after light irradiation, suggesting that the membrane disruption is possibly caused by oxidation of membrane lipids with ROS generated from photosensitizers and affects the photo-cytotoxicity in PDT.

KW - Drug delivery system (DDS)

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KW - Photodynamic therapy (PDT)

KW - Protoporphyrin IX (PPIX)

KW - Reactive oxygen species (ROS)

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Localization-dependent cell-killing effects of protoporphyrin (PPIX)-lipid micelles and liposomes in photodynamic therapy

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Keywords

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