TY - JOUR
T1 - Low molecular weight immunosuppressive factors found in elevated amounts in cancer ascitic fluids of mice 1. Isolation, identification and immunosuppressive effects of uric acid and uracil
AU - Saim, Shunsuke
AU - Takano, Shoichi
AU - Majima, Toshiro
AU - Aso, Hisashi
AU - Nakamura, Takehiko
AU - Ishida, Nakao
PY - 1986/1/1
Y1 - 1986/1/1
N2 - A definite increase in two low molecular weight factors, G10-2 and G10-3 was found in Ehrlich ascitic fluids, parallel to tumor growth. The isolation and identification of the two factors were attempted through gel filtration and reversed phase column chromatography, using ascitic fluids obtained 13 days after intraperitoneal implantation of Ehrlich tumor cells. As a result, two highly purified factors were observed upon examination by high performance liquid chromatography. Additional analytical data, collected by UV spectrum, NMR spectrum and mass analysis. allowed us to identify G10-2 as uric acid and G10-3 as uracil. Detailed immunological analysis of uric acid and uracil revealed that the augmenting activities of mouse and human NK cells by mouse IFNδbeta; or human rIFN δA/D were impaired in the presence of either compound at concentrations of 0.07 mM, the concentration detectable in the ascitic fluid of tumor bearing mice.
AB - A definite increase in two low molecular weight factors, G10-2 and G10-3 was found in Ehrlich ascitic fluids, parallel to tumor growth. The isolation and identification of the two factors were attempted through gel filtration and reversed phase column chromatography, using ascitic fluids obtained 13 days after intraperitoneal implantation of Ehrlich tumor cells. As a result, two highly purified factors were observed upon examination by high performance liquid chromatography. Additional analytical data, collected by UV spectrum, NMR spectrum and mass analysis. allowed us to identify G10-2 as uric acid and G10-3 as uracil. Detailed immunological analysis of uric acid and uracil revealed that the augmenting activities of mouse and human NK cells by mouse IFNδbeta; or human rIFN δA/D were impaired in the presence of either compound at concentrations of 0.07 mM, the concentration detectable in the ascitic fluid of tumor bearing mice.
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U2 - 10.3109/08923978609031084
DO - 10.3109/08923978609031084
M3 - Article
C2 - 3711673
AN - SCOPUS:84907112628
SN - 0892-3973
VL - 8
SP - 39
EP - 58
JO - Immunopharmacology and Immunotoxicology
JF - Immunopharmacology and Immunotoxicology
IS - 1
ER -
