LSI-based amperometric sensor for real-time monitoring of embryoid bodies

Mustafa Şen; Kosuke Ino; Kumi Y. Inoue; Toshiharu Arai; Taku Nishijo; Atsushi Suda; Ryota Kunikata; Hitoshi Shiku; Tomokazu Matsue

doi:10.1016/j.bios.2013.03.069

LSI-based amperometric sensor for real-time monitoring of embryoid bodies

Mustafa Şen, Kosuke Ino, Kumi Y. Inoue, Toshiharu Arai, Taku Nishijo, Atsushi Suda, Ryota Kunikata, Hitoshi Shiku, Tomokazu Matsue

Research output: Contribution to journal › Article › peer-review

47 Citations (Scopus)

Abstract

A large scale integration (LSI)-based amperometric sensor is used for electrochemical evaluation and real-time monitoring of the alkaline phosphatase (ALP) activity of mouse embryoid bodies (EBs). EBs were prepared by the hanging drop culture of embryonic stem (ES) cells. The ALP activity of EBs with various sizes was electrochemically detected at 400 measurement points on a Bio-LSI chip. The electrochemical measurements revealed that the relative ALP activity was low for large EBs and decreased with progress of the differentiation level of the ES cells. The ALP activity of the EBs was successfully monitored in real time for 3.5 h, and their ALP activity in a glucose-free buffer decreased after 2 h. To the best of our knowledge, this is the first report on the application of an LSI-based amperometric sensor for real-time cell monitoring over 3 h. The chip is expected to be useful for the evaluation of cell activities.

Original language	English
Pages (from-to)	12-18
Number of pages	7
Journal	Biosensors and Bioelectronics
Volume	48
DOIs	https://doi.org/10.1016/j.bios.2013.03.069
Publication status	Published - 2013 Oct 5

Keywords

Cell analysis
Electrochemistry
Embryoid body
LSI-based electrochemical detection
Real-time monitoring

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10.1016/j.bios.2013.03.069

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@article{fda359459aa445918ae260d67e82c4e2,

title = "LSI-based amperometric sensor for real-time monitoring of embryoid bodies",

abstract = "A large scale integration (LSI)-based amperometric sensor is used for electrochemical evaluation and real-time monitoring of the alkaline phosphatase (ALP) activity of mouse embryoid bodies (EBs). EBs were prepared by the hanging drop culture of embryonic stem (ES) cells. The ALP activity of EBs with various sizes was electrochemically detected at 400 measurement points on a Bio-LSI chip. The electrochemical measurements revealed that the relative ALP activity was low for large EBs and decreased with progress of the differentiation level of the ES cells. The ALP activity of the EBs was successfully monitored in real time for 3.5 h, and their ALP activity in a glucose-free buffer decreased after 2 h. To the best of our knowledge, this is the first report on the application of an LSI-based amperometric sensor for real-time cell monitoring over 3 h. The chip is expected to be useful for the evaluation of cell activities.",

keywords = "Cell analysis, Electrochemistry, Embryoid body, LSI-based electrochemical detection, Real-time monitoring",

author = "Mustafa {\c S}en and Kosuke Ino and Inoue, {Kumi Y.} and Toshiharu Arai and Taku Nishijo and Atsushi Suda and Ryota Kunikata and Hitoshi Shiku and Tomokazu Matsue",

note = "Funding Information: This work was supported in part by a Grant-in-Aid for Scientific Research (A) (No. 22245011 ) and a Grant-in-Aid for Young Scientists (B) (No. 23760745 ) from the Japan Society for the Promotion of Science (JSPS) . This research was partly supported by Special Coordination Funds for Promoting Science and Technology , Creation of Innovation Centers for Advanced Interdisciplinary Research Areas Program from the Japan Science and Technology Agency . The authors also thank Prof. Izumi Kumagai and Dr. Ryutaro Asano for allowing us to use the equipment for chemiluminescence detection. ",

year = "2013",

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doi = "10.1016/j.bios.2013.03.069",

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AU - Şen, Mustafa

AU - Ino, Kosuke

AU - Inoue, Kumi Y.

AU - Arai, Toshiharu

AU - Nishijo, Taku

AU - Suda, Atsushi

AU - Kunikata, Ryota

AU - Shiku, Hitoshi

AU - Matsue, Tomokazu

N1 - Funding Information: This work was supported in part by a Grant-in-Aid for Scientific Research (A) (No. 22245011 ) and a Grant-in-Aid for Young Scientists (B) (No. 23760745 ) from the Japan Society for the Promotion of Science (JSPS) . This research was partly supported by Special Coordination Funds for Promoting Science and Technology , Creation of Innovation Centers for Advanced Interdisciplinary Research Areas Program from the Japan Science and Technology Agency . The authors also thank Prof. Izumi Kumagai and Dr. Ryutaro Asano for allowing us to use the equipment for chemiluminescence detection.

PY - 2013/10/5

Y1 - 2013/10/5

N2 - A large scale integration (LSI)-based amperometric sensor is used for electrochemical evaluation and real-time monitoring of the alkaline phosphatase (ALP) activity of mouse embryoid bodies (EBs). EBs were prepared by the hanging drop culture of embryonic stem (ES) cells. The ALP activity of EBs with various sizes was electrochemically detected at 400 measurement points on a Bio-LSI chip. The electrochemical measurements revealed that the relative ALP activity was low for large EBs and decreased with progress of the differentiation level of the ES cells. The ALP activity of the EBs was successfully monitored in real time for 3.5 h, and their ALP activity in a glucose-free buffer decreased after 2 h. To the best of our knowledge, this is the first report on the application of an LSI-based amperometric sensor for real-time cell monitoring over 3 h. The chip is expected to be useful for the evaluation of cell activities.

AB - A large scale integration (LSI)-based amperometric sensor is used for electrochemical evaluation and real-time monitoring of the alkaline phosphatase (ALP) activity of mouse embryoid bodies (EBs). EBs were prepared by the hanging drop culture of embryonic stem (ES) cells. The ALP activity of EBs with various sizes was electrochemically detected at 400 measurement points on a Bio-LSI chip. The electrochemical measurements revealed that the relative ALP activity was low for large EBs and decreased with progress of the differentiation level of the ES cells. The ALP activity of the EBs was successfully monitored in real time for 3.5 h, and their ALP activity in a glucose-free buffer decreased after 2 h. To the best of our knowledge, this is the first report on the application of an LSI-based amperometric sensor for real-time cell monitoring over 3 h. The chip is expected to be useful for the evaluation of cell activities.

KW - Cell analysis

KW - Electrochemistry

KW - Embryoid body

KW - LSI-based electrochemical detection

KW - Real-time monitoring

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LSI-based amperometric sensor for real-time monitoring of embryoid bodies

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Keywords

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