LSI-based amperometric sensor for real-time monitoring of embryoid bodies

Mustafa Şen, Kosuke Ino, Kumi Y. Inoue, Toshiharu Arai, Taku Nishijo, Atsushi Suda, Ryota Kunikata, Hitoshi Shiku, Tomokazu Matsue

Research output: Contribution to journalArticlepeer-review

47 Citations (Scopus)


A large scale integration (LSI)-based amperometric sensor is used for electrochemical evaluation and real-time monitoring of the alkaline phosphatase (ALP) activity of mouse embryoid bodies (EBs). EBs were prepared by the hanging drop culture of embryonic stem (ES) cells. The ALP activity of EBs with various sizes was electrochemically detected at 400 measurement points on a Bio-LSI chip. The electrochemical measurements revealed that the relative ALP activity was low for large EBs and decreased with progress of the differentiation level of the ES cells. The ALP activity of the EBs was successfully monitored in real time for 3.5 h, and their ALP activity in a glucose-free buffer decreased after 2 h. To the best of our knowledge, this is the first report on the application of an LSI-based amperometric sensor for real-time cell monitoring over 3 h. The chip is expected to be useful for the evaluation of cell activities.

Original languageEnglish
Pages (from-to)12-18
Number of pages7
JournalBiosensors and Bioelectronics
Publication statusPublished - 2013 Oct 5


  • Cell analysis
  • Electrochemistry
  • Embryoid body
  • LSI-based electrochemical detection
  • Real-time monitoring


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