Luminol chemiluminescent determination of hydrogen peroxide at picomole levels using high-performance liquid chromatography with a cation-exchange resin gel column

Teruo Miyazawa; Sittiwat Lertsiri; Kenshiro Fujimoto; Michiko Oka

doi:10.1016/0021-9673(94)89056-0

Luminol chemiluminescent determination of hydrogen peroxide at picomole levels using high-performance liquid chromatography with a cation-exchange resin gel column

Teruo Miyazawa, Sittiwat Lertsiri, Kenshiro Fujimoto, Michiko Oka

New Industry Creation Hatchery Center (NICHe)

Tohoku University

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

The optimum conditions of high-performance liquid chromatography combined with luminol chemiluminescence detection were established for the determination of hydrogen peroxide at picomole levels using a cation-exchange gel column. The gel column with distilled water as the mobile phase allowed a good separation of H₂O₂ without causing any irreversible binding of H₂O₂ to the column surface. The detection limit and the quantification limit of H₂O₂ were 4 and 6-600 pmol, respectively. The suitability of the present method was verified by the determination of H₂O₂ present in coffee drinks.

Original language	English
Pages (from-to)	99-104
Number of pages	6
Journal	Journal of Chromatography A
Volume	667
Issue number	1-2
DOIs	https://doi.org/10.1016/0021-9673(94)89056-0
Publication status	Published - 1994 Apr 29

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10.1016/0021-9673(94)89056-0

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title = "Luminol chemiluminescent determination of hydrogen peroxide at picomole levels using high-performance liquid chromatography with a cation-exchange resin gel column",

abstract = "The optimum conditions of high-performance liquid chromatography combined with luminol chemiluminescence detection were established for the determination of hydrogen peroxide at picomole levels using a cation-exchange gel column. The gel column with distilled water as the mobile phase allowed a good separation of H2O2 without causing any irreversible binding of H2O2 to the column surface. The detection limit and the quantification limit of H2O2 were 4 and 6-600 pmol, respectively. The suitability of the present method was verified by the determination of H2O2 present in coffee drinks.",

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Luminol chemiluminescent determination of hydrogen peroxide at picomole levels using high-performance liquid chromatography with a cation-exchange resin gel column. / Miyazawa, Teruo; Lertsiri, Sittiwat; Fujimoto, Kenshiro et al.
In: Journal of Chromatography A, Vol. 667, No. 1-2, 29.04.1994, p. 99-104.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Luminol chemiluminescent determination of hydrogen peroxide at picomole levels using high-performance liquid chromatography with a cation-exchange resin gel column

AU - Miyazawa, Teruo

AU - Lertsiri, Sittiwat

AU - Fujimoto, Kenshiro

AU - Oka, Michiko

PY - 1994/4/29

Y1 - 1994/4/29

N2 - The optimum conditions of high-performance liquid chromatography combined with luminol chemiluminescence detection were established for the determination of hydrogen peroxide at picomole levels using a cation-exchange gel column. The gel column with distilled water as the mobile phase allowed a good separation of H2O2 without causing any irreversible binding of H2O2 to the column surface. The detection limit and the quantification limit of H2O2 were 4 and 6-600 pmol, respectively. The suitability of the present method was verified by the determination of H2O2 present in coffee drinks.

AB - The optimum conditions of high-performance liquid chromatography combined with luminol chemiluminescence detection were established for the determination of hydrogen peroxide at picomole levels using a cation-exchange gel column. The gel column with distilled water as the mobile phase allowed a good separation of H2O2 without causing any irreversible binding of H2O2 to the column surface. The detection limit and the quantification limit of H2O2 were 4 and 6-600 pmol, respectively. The suitability of the present method was verified by the determination of H2O2 present in coffee drinks.

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Luminol chemiluminescent determination of hydrogen peroxide at picomole levels using high-performance liquid chromatography with a cation-exchange resin gel column

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