@article{c2e2c953e6344458a48ea38949da434c,
title = "Lysophosphatidylcholine generates superoxide anions through activation of phosphatidylinositol 3-kinase in human neutrophils",
abstract = "Lysophosphatidylcholine (LPC) accumulates in inflammatory tissues, where neutrophils are recruited to generate superoxide anions (O(·-)2). Here, we show that LPC stimulates O(·-)2 generation in human neutrophils and that the activity is inhibited with phosphatidylinositol 3-kinase (PI3 kinase) inhibitors, but not with protein kinase C (PKC) inhibitors. Furthermore, we demonstrate that LPC activates PI3 kinase in neutrophils. Thus, LPC might contribute to host defense by generating O(·-)2 in neutrophils through PI3 kinase activation, but not through PKC activation. Copyright (C) 1998 Federation of European Biochemical Societies.",
keywords = "Lysophosphatidylcholine, Neutrophil, Phosphatidylinositol 3-kinase, Protein kinase C, Superoxide anion",
author = "Hiroaki Nishioka and Hisanori Horiuchi and Hidenori Arai and Toru Kita",
note = "Funding Information: We thank Dr. S. Ando (Fujirebio, Hachioji, Japan) and Dr. Y. Minamiyama (Osaka City University, Japan) for advice for the assay of O ⋅− 2 generation, and Dr. H. Ozaki (Kyoto University, Japan) for helpful discussion, Dr. H. Stenmark (The Norwegian Radium Hospital, Oslo, Norway), Dr. H. McBride (European Molecular Biology Laboratory, Heidelberg, Germany), Dr. N. Kume and Dr. H. Kataoka (Kyoto University) for critical reading of the manuscript. This work was supported by Research Grants No. 09780571, 90291426 (H.H.), 08407026, 09044293, 09281104, 09281103, and 09NP0601 (T.K.) from the Ministry of Education, Science, Sports and Culture, Japan, and Research Grants for Cardiovascular Diseases (A8-1) (T.K.), Research Grants for Surveys and Research on Specific Diseases (T.K.), and Special Research (H10-Special-012) of Health Science Research Grant (H.H.) from the Ministry of Health and Welfare, Japan. This work was also supported in part by the Research Grants from the Novartis Foundation (Japan) for the Promotion of Science (H.H.) and from Japan Intractable Diseases Research Foundation (H.H.).",
year = "1998",
month = dec,
day = "11",
doi = "10.1016/S0014-5793(98)01526-9",
language = "English",
volume = "441",
pages = "63--66",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Wiley-Blackwell",
number = "1",
}