TY - GEN
T1 - Macrophage with gold nanorod visualized by optical-resolution and acoustic-resolution photoacoustic microscopes
AU - Yamazaki, Rena
AU - Ogasawara, Koetsu
AU - Fujiwara, Mitsuhiro
AU - Kobayashi, Kazuto
AU - Saijo, Yoshifumi
N1 - Publisher Copyright:
© 2015 IEEE.
PY - 2015/11/4
Y1 - 2015/11/4
N2 - Macrophages play a key role in inflammation and they are frequently observed in vulnerable atherosclerotic plaque. In the present study, macrophages phagocytosing gold nanorod (AuNR) were observed by optical-resolution (OR) and acoustic-resolution (AR) photoacoustic microscope (PAM). The OR-PAM consisted of diode laser optically focused to 60 micron and planar ultrasonic transducer with the central frequency of 8 MHz placed under the object. AR-PAM consisted of concave ultrasonic transducer with the central frequency of 20 MHz and optical fiber through the center hole of the transducer for laser irradiation. First, PA signal from gold, silver and copper wire were measured in order to determine the best metal substrate for enhancing PA contrast. Gold generated largest PA signal. AuNR with the resonance wavelength of 1064 nm was co-cultured with the macrophages for phagocytosis. PA signal was successfully detected from macrophages with AuNR by both OR-PAM and AR-PAM. PA imaging of the macrophages with AuNR indicates inflammation in the vulnerable plaque and AR-PAM method would be applicable for clinical settings.
AB - Macrophages play a key role in inflammation and they are frequently observed in vulnerable atherosclerotic plaque. In the present study, macrophages phagocytosing gold nanorod (AuNR) were observed by optical-resolution (OR) and acoustic-resolution (AR) photoacoustic microscope (PAM). The OR-PAM consisted of diode laser optically focused to 60 micron and planar ultrasonic transducer with the central frequency of 8 MHz placed under the object. AR-PAM consisted of concave ultrasonic transducer with the central frequency of 20 MHz and optical fiber through the center hole of the transducer for laser irradiation. First, PA signal from gold, silver and copper wire were measured in order to determine the best metal substrate for enhancing PA contrast. Gold generated largest PA signal. AuNR with the resonance wavelength of 1064 nm was co-cultured with the macrophages for phagocytosis. PA signal was successfully detected from macrophages with AuNR by both OR-PAM and AR-PAM. PA imaging of the macrophages with AuNR indicates inflammation in the vulnerable plaque and AR-PAM method would be applicable for clinical settings.
UR - http://www.scopus.com/inward/record.url?scp=84953236813&partnerID=8YFLogxK
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U2 - 10.1109/EMBC.2015.7318874
DO - 10.1109/EMBC.2015.7318874
M3 - Conference contribution
C2 - 26736774
AN - SCOPUS:84953236813
T3 - Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS
SP - 2387
EP - 2390
BT - 2015 37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2015
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2015
Y2 - 25 August 2015 through 29 August 2015
ER -