Mass fabrication of small cell spheroids by using micro-patterned tissue culture plate

Akinari Iwasaki; Takuya Matsumoto; Go Tazaki; Hitoshi Tsuruta; Hiroshi Egusa; Hiroyuki Miyajima; Taiji Sohmura

doi:10.1002/adem.200800392

Mass fabrication of small cell spheroids by using micro-patterned tissue culture plate

Akinari Iwasaki, Takuya Matsumoto, Go Tazaki, Hitoshi Tsuruta, Hiroshi Egusa, Hiroyuki Miyajima, Taiji Sohmura

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

A newly designed micro-patterned chamber was utilized to fabricate cell spheroids with a constant size (<200μm) and cell number. By applying cytochalasin D as a chemical to control cell adhesion and aggregation, thousands of aggregated cells were formed in each patterned chamber. Importantly, the formed cell spheroids were collected by a simple pipetting process without using proteinase.

Original language	English
Pages (from-to)	801-804
Number of pages	4
Journal	Advanced Engineering Materials
Volume	11
Issue number	10
DOIs	https://doi.org/10.1002/adem.200800392
Publication status	Published - 2009 Oct
Externally published	Yes

ASJC Scopus subject areas

Materials Science(all)
Condensed Matter Physics

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10.1002/adem.200800392

Cite this

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abstract = "A newly designed micro-patterned chamber was utilized to fabricate cell spheroids with a constant size (<200μm) and cell number. By applying cytochalasin D as a chemical to control cell adhesion and aggregation, thousands of aggregated cells were formed in each patterned chamber. Importantly, the formed cell spheroids were collected by a simple pipetting process without using proteinase.",

author = "Akinari Iwasaki and Takuya Matsumoto and Go Tazaki and Hitoshi Tsuruta and Hiroshi Egusa and Hiroyuki Miyajima and Taiji Sohmura",

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AU - Iwasaki, Akinari

AU - Matsumoto, Takuya

AU - Tazaki, Go

AU - Tsuruta, Hitoshi

AU - Egusa, Hiroshi

AU - Miyajima, Hiroyuki

AU - Sohmura, Taiji

PY - 2009/10

Y1 - 2009/10

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AB - A newly designed micro-patterned chamber was utilized to fabricate cell spheroids with a constant size (<200μm) and cell number. By applying cytochalasin D as a chemical to control cell adhesion and aggregation, thousands of aggregated cells were formed in each patterned chamber. Importantly, the formed cell spheroids were collected by a simple pipetting process without using proteinase.

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Mass fabrication of small cell spheroids by using micro-patterned tissue culture plate

Abstract

ASJC Scopus subject areas

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